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Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales
Agouti-related-peptide (AgRP) neurons—interoceptive neurons in the arcuate nucleus of the hypothalamus (ARC)—are both necessary and sufficient for driving feeding behavior. To better understand the functional roles of AgRP neurons, we performed optetrode electrophysiological recordings from AgRP neu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498165/ https://www.ncbi.nlm.nih.gov/pubmed/26159614 http://dx.doi.org/10.7554/eLife.07122 |
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author | Mandelblat-Cerf, Yael Ramesh, Rohan N Burgess, Christian R Patella, Paola Yang, Zongfang Lowell, Bradford B Andermann, Mark L |
author_facet | Mandelblat-Cerf, Yael Ramesh, Rohan N Burgess, Christian R Patella, Paola Yang, Zongfang Lowell, Bradford B Andermann, Mark L |
author_sort | Mandelblat-Cerf, Yael |
collection | PubMed |
description | Agouti-related-peptide (AgRP) neurons—interoceptive neurons in the arcuate nucleus of the hypothalamus (ARC)—are both necessary and sufficient for driving feeding behavior. To better understand the functional roles of AgRP neurons, we performed optetrode electrophysiological recordings from AgRP neurons in awake, behaving AgRP-IRES-Cre mice. In free-feeding mice, we observed a fivefold increase in AgRP neuron firing with mounting caloric deficit in afternoon vs morning recordings. In food-restricted mice, as food became available, AgRP neuron firing dropped, yet remained elevated as compared to firing in sated mice. The rapid drop in spiking activity of AgRP neurons at meal onset may reflect a termination of the drive to find food, while residual, persistent spiking may reflect a sustained drive to consume food. Moreover, nearby neurons inhibited by AgRP neuron photostimulation, likely including satiety-promoting pro-opiomelanocortin (POMC) neurons, demonstrated opposite changes in spiking. Finally, firing of ARC neurons was also rapidly modulated within seconds of individual licks for liquid food. These findings suggest novel roles for antagonistic AgRP and POMC neurons in the regulation of feeding behaviors across multiple timescales. DOI: http://dx.doi.org/10.7554/eLife.07122.001 |
format | Online Article Text |
id | pubmed-4498165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44981652015-07-11 Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales Mandelblat-Cerf, Yael Ramesh, Rohan N Burgess, Christian R Patella, Paola Yang, Zongfang Lowell, Bradford B Andermann, Mark L eLife Neuroscience Agouti-related-peptide (AgRP) neurons—interoceptive neurons in the arcuate nucleus of the hypothalamus (ARC)—are both necessary and sufficient for driving feeding behavior. To better understand the functional roles of AgRP neurons, we performed optetrode electrophysiological recordings from AgRP neurons in awake, behaving AgRP-IRES-Cre mice. In free-feeding mice, we observed a fivefold increase in AgRP neuron firing with mounting caloric deficit in afternoon vs morning recordings. In food-restricted mice, as food became available, AgRP neuron firing dropped, yet remained elevated as compared to firing in sated mice. The rapid drop in spiking activity of AgRP neurons at meal onset may reflect a termination of the drive to find food, while residual, persistent spiking may reflect a sustained drive to consume food. Moreover, nearby neurons inhibited by AgRP neuron photostimulation, likely including satiety-promoting pro-opiomelanocortin (POMC) neurons, demonstrated opposite changes in spiking. Finally, firing of ARC neurons was also rapidly modulated within seconds of individual licks for liquid food. These findings suggest novel roles for antagonistic AgRP and POMC neurons in the regulation of feeding behaviors across multiple timescales. DOI: http://dx.doi.org/10.7554/eLife.07122.001 eLife Sciences Publications, Ltd 2015-07-10 /pmc/articles/PMC4498165/ /pubmed/26159614 http://dx.doi.org/10.7554/eLife.07122 Text en © 2015, Mandelblat-Cerf et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Mandelblat-Cerf, Yael Ramesh, Rohan N Burgess, Christian R Patella, Paola Yang, Zongfang Lowell, Bradford B Andermann, Mark L Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title | Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title_full | Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title_fullStr | Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title_full_unstemmed | Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title_short | Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales |
title_sort | arcuate hypothalamic agrp and putative pomc neurons show opposite changes in spiking across multiple timescales |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498165/ https://www.ncbi.nlm.nih.gov/pubmed/26159614 http://dx.doi.org/10.7554/eLife.07122 |
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