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Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats

The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery oc...

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Detalles Bibliográficos
Autores principales: Li, Xin-juan, Li, Chao-kun, Wei, Lin-yu, Lu, Na, Wang, Guo-hong, Zhao, Hong-gang, Li, Dong-liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498355/
https://www.ncbi.nlm.nih.gov/pubmed/26199610
http://dx.doi.org/10.4103/1673-5374.158353
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author Li, Xin-juan
Li, Chao-kun
Wei, Lin-yu
Lu, Na
Wang, Guo-hong
Zhao, Hong-gang
Li, Dong-liang
author_facet Li, Xin-juan
Li, Chao-kun
Wei, Lin-yu
Lu, Na
Wang, Guo-hong
Zhao, Hong-gang
Li, Dong-liang
author_sort Li, Xin-juan
collection PubMed
description The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery occlusion, the animals were intraperitoneally injected with hydrogen sulfide donor compound sodium hydrosulfide. Immunofluorescence revealed that the immunoreactivity of P2X(7) in the cerebral cortex and hippocampal CA1 region in rats with cerebral ischemia/reperfusion injury decreased with hydrogen sulfide treatment. Furthermore, treatment of these rats with hydrogen sulfide significantly lowered mortality, the Longa neurological deficit scores, and infarct volume. These results indicate that hydrogen sulfide may be protective in rats with local cerebral ischemia/reperfusion injury by down-regulating the expression of P2X(7) receptors.
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spelling pubmed-44983552015-07-21 Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats Li, Xin-juan Li, Chao-kun Wei, Lin-yu Lu, Na Wang, Guo-hong Zhao, Hong-gang Li, Dong-liang Neural Regen Res Research Article The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery occlusion, the animals were intraperitoneally injected with hydrogen sulfide donor compound sodium hydrosulfide. Immunofluorescence revealed that the immunoreactivity of P2X(7) in the cerebral cortex and hippocampal CA1 region in rats with cerebral ischemia/reperfusion injury decreased with hydrogen sulfide treatment. Furthermore, treatment of these rats with hydrogen sulfide significantly lowered mortality, the Longa neurological deficit scores, and infarct volume. These results indicate that hydrogen sulfide may be protective in rats with local cerebral ischemia/reperfusion injury by down-regulating the expression of P2X(7) receptors. Medknow Publications & Media Pvt Ltd 2015-06 /pmc/articles/PMC4498355/ /pubmed/26199610 http://dx.doi.org/10.4103/1673-5374.158353 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Xin-juan
Li, Chao-kun
Wei, Lin-yu
Lu, Na
Wang, Guo-hong
Zhao, Hong-gang
Li, Dong-liang
Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title_full Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title_fullStr Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title_full_unstemmed Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title_short Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
title_sort hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498355/
https://www.ncbi.nlm.nih.gov/pubmed/26199610
http://dx.doi.org/10.4103/1673-5374.158353
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