Cargando…

Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle

Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy,...

Descripción completa

Detalles Bibliográficos
Autores principales: ABOELENAIN, Mansour, KAWAHARA, Manabu, BALBOULA, Ahmed Zaky, MONTASSER, Abd El-monem, ZAABEL, Samy Mowaed, OKUDA, Kiyoshi, TAKAHASHI, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498366/
https://www.ncbi.nlm.nih.gov/pubmed/25819401
http://dx.doi.org/10.1262/jrd.2014-135
_version_ 1782380616349646848
author ABOELENAIN, Mansour
KAWAHARA, Manabu
BALBOULA, Ahmed Zaky
MONTASSER, Abd El-monem
ZAABEL, Samy Mowaed
OKUDA, Kiyoshi
TAKAHASHI, Masashi
author_facet ABOELENAIN, Mansour
KAWAHARA, Manabu
BALBOULA, Ahmed Zaky
MONTASSER, Abd El-monem
ZAABEL, Samy Mowaed
OKUDA, Kiyoshi
TAKAHASHI, Masashi
author_sort ABOELENAIN, Mansour
collection PubMed
description Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.
format Online
Article
Text
id pubmed-4498366
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Society for Reproduction and Development
record_format MEDLINE/PubMed
spelling pubmed-44983662015-07-13 Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle ABOELENAIN, Mansour KAWAHARA, Manabu BALBOULA, Ahmed Zaky MONTASSER, Abd El-monem ZAABEL, Samy Mowaed OKUDA, Kiyoshi TAKAHASHI, Masashi J Reprod Dev Original Article Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL. The Society for Reproduction and Development 2015-03-28 2015-06 /pmc/articles/PMC4498366/ /pubmed/25819401 http://dx.doi.org/10.1262/jrd.2014-135 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
ABOELENAIN, Mansour
KAWAHARA, Manabu
BALBOULA, Ahmed Zaky
MONTASSER, Abd El-monem
ZAABEL, Samy Mowaed
OKUDA, Kiyoshi
TAKAHASHI, Masashi
Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title_full Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title_fullStr Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title_full_unstemmed Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title_short Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
title_sort status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498366/
https://www.ncbi.nlm.nih.gov/pubmed/25819401
http://dx.doi.org/10.1262/jrd.2014-135
work_keys_str_mv AT aboelenainmansour statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT kawaharamanabu statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT balboulaahmedzaky statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT montasserabdelmonem statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT zaabelsamymowaed statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT okudakiyoshi statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle
AT takahashimasashi statusofautophagylysosomeactivityandapoptosisduringcorpusluteumregressionincattle