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Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development

Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplanta...

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Autores principales: OOGA, Masatoshi, SUZUKI, Masataka G., AOKI, Fugaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498372/
https://www.ncbi.nlm.nih.gov/pubmed/25740253
http://dx.doi.org/10.1262/jrd.2014-137
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author OOGA, Masatoshi
SUZUKI, Masataka G.
AOKI, Fugaku
author_facet OOGA, Masatoshi
SUZUKI, Masataka G.
AOKI, Fugaku
author_sort OOGA, Masatoshi
collection PubMed
description Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development.
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spelling pubmed-44983722015-07-13 Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development OOGA, Masatoshi SUZUKI, Masataka G. AOKI, Fugaku J Reprod Dev Original Article Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development. The Society for Reproduction and Development 2015-02-06 2015-06 /pmc/articles/PMC4498372/ /pubmed/25740253 http://dx.doi.org/10.1262/jrd.2014-137 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
OOGA, Masatoshi
SUZUKI, Masataka G.
AOKI, Fugaku
Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_full Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_fullStr Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_full_unstemmed Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_short Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_sort involvement of histone h2b monoubiquitination in the regulation of mouse preimplantation development
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498372/
https://www.ncbi.nlm.nih.gov/pubmed/25740253
http://dx.doi.org/10.1262/jrd.2014-137
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