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Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes

The capability of Vibrio alginolyticus to adhere to fish mucus is a key virulence factor of the bacteria. Our previous research showed that stress conditions, such as Cu(2+), Pb(2+), Hg(2+), and low pH, can reduce this adhesion ability. Non-coding (nc) RNAs play a crucial role in regulating bacteria...

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Autores principales: Huang, Lixing, Hu, Jiao, Su, Yongquan, Qin, Yingxue, Kong, Wendi, Ma, Ying, Xu, Xiaojin, Lin, Mao, Yan, Qingpi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498440/
https://www.ncbi.nlm.nih.gov/pubmed/26217589
http://dx.doi.org/10.3389/fcimb.2015.00056
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author Huang, Lixing
Hu, Jiao
Su, Yongquan
Qin, Yingxue
Kong, Wendi
Ma, Ying
Xu, Xiaojin
Lin, Mao
Yan, Qingpi
author_facet Huang, Lixing
Hu, Jiao
Su, Yongquan
Qin, Yingxue
Kong, Wendi
Ma, Ying
Xu, Xiaojin
Lin, Mao
Yan, Qingpi
author_sort Huang, Lixing
collection PubMed
description The capability of Vibrio alginolyticus to adhere to fish mucus is a key virulence factor of the bacteria. Our previous research showed that stress conditions, such as Cu(2+), Pb(2+), Hg(2+), and low pH, can reduce this adhesion ability. Non-coding (nc) RNAs play a crucial role in regulating bacterial gene expression, affecting the bacteria's pathogenicity. To investigate the mechanism(s) underlying the decline in adhesion ability caused by stressors, we combined high-throughput sequencing with computational techniques to detect stressed ncRNA dynamics. These approaches yielded three commonly altered ncRNAs that are predicted to regulate the bacterial chemotaxis pathway, which plays a key role in the adhesion process of bacteria. We hypothesized they play a key role in the adhesion process of V. alginolyticus. In this study, we validated the effects of these three ncRNAs on their predicted target genes and their role in the V. alginolyticus adhesion process with RNA interference (i), quantitative real-time polymerase chain reaction (qPCR), northern blot, capillary assay, and in vitro adhesion assays. The expression of these ncRNAs and their predicted target genes were confirmed by qPCR and northern blot, which reinforced the reliability of the sequencing data and the target prediction. Overexpression of these ncRNAs was capable of reducing the chemotactic and adhesion ability of V. alginolyticus, and the expression levels of their target genes were also significantly reduced. Our results indicated that these three ncRNAs: (1) are able to regulate the bacterial chemotaxis pathway, and (2) play a key role in the adhesion process of V. alginolyticus.
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spelling pubmed-44984402015-07-27 Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes Huang, Lixing Hu, Jiao Su, Yongquan Qin, Yingxue Kong, Wendi Ma, Ying Xu, Xiaojin Lin, Mao Yan, Qingpi Front Cell Infect Microbiol Microbiology The capability of Vibrio alginolyticus to adhere to fish mucus is a key virulence factor of the bacteria. Our previous research showed that stress conditions, such as Cu(2+), Pb(2+), Hg(2+), and low pH, can reduce this adhesion ability. Non-coding (nc) RNAs play a crucial role in regulating bacterial gene expression, affecting the bacteria's pathogenicity. To investigate the mechanism(s) underlying the decline in adhesion ability caused by stressors, we combined high-throughput sequencing with computational techniques to detect stressed ncRNA dynamics. These approaches yielded three commonly altered ncRNAs that are predicted to regulate the bacterial chemotaxis pathway, which plays a key role in the adhesion process of bacteria. We hypothesized they play a key role in the adhesion process of V. alginolyticus. In this study, we validated the effects of these three ncRNAs on their predicted target genes and their role in the V. alginolyticus adhesion process with RNA interference (i), quantitative real-time polymerase chain reaction (qPCR), northern blot, capillary assay, and in vitro adhesion assays. The expression of these ncRNAs and their predicted target genes were confirmed by qPCR and northern blot, which reinforced the reliability of the sequencing data and the target prediction. Overexpression of these ncRNAs was capable of reducing the chemotactic and adhesion ability of V. alginolyticus, and the expression levels of their target genes were also significantly reduced. Our results indicated that these three ncRNAs: (1) are able to regulate the bacterial chemotaxis pathway, and (2) play a key role in the adhesion process of V. alginolyticus. Frontiers Media S.A. 2015-07-10 /pmc/articles/PMC4498440/ /pubmed/26217589 http://dx.doi.org/10.3389/fcimb.2015.00056 Text en Copyright © 2015 Huang, Hu, Su, Qin, Kong, Ma, Xu, Lin and Yan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Lixing
Hu, Jiao
Su, Yongquan
Qin, Yingxue
Kong, Wendi
Ma, Ying
Xu, Xiaojin
Lin, Mao
Yan, Qingpi
Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title_full Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title_fullStr Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title_full_unstemmed Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title_short Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes
title_sort identification and characterization of three vibrio alginolyticus non-coding rnas involved in adhesion, chemotaxis, and motility processes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498440/
https://www.ncbi.nlm.nih.gov/pubmed/26217589
http://dx.doi.org/10.3389/fcimb.2015.00056
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