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The MORPHEUS II protein crystallization screen
High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen fo...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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International Union of Crystallography
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498703/ https://www.ncbi.nlm.nih.gov/pubmed/26144227 http://dx.doi.org/10.1107/S2053230X1500967X |
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author | Gorrec, Fabrice |
author_facet | Gorrec, Fabrice |
author_sort | Gorrec, Fabrice |
collection | PubMed |
description | High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen formulations are continuously being developed. In the past, MORPHEUS, a screen in which each condition integrates a mix of additives selected from the Protein Data Bank, a cryoprotectant and a buffer system, was developed. Here, MORPHEUS II, a follow-up to the original 96-condition initial screen, is described. Reagents were selected to yield crystals when none might be observed in traditional initial screens. Besides, the screen includes heavy atoms for experimental phasing and small polyols to ensure the cryoprotection of crystals. The suitability of the resulting novel conditions is shown by the crystallization of a broad variety of protein samples and their efficiency is compared with commercially available conditions. |
format | Online Article Text |
id | pubmed-4498703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-44987032015-07-28 The MORPHEUS II protein crystallization screen Gorrec, Fabrice Acta Crystallogr F Struct Biol Commun Iccbm15 High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen formulations are continuously being developed. In the past, MORPHEUS, a screen in which each condition integrates a mix of additives selected from the Protein Data Bank, a cryoprotectant and a buffer system, was developed. Here, MORPHEUS II, a follow-up to the original 96-condition initial screen, is described. Reagents were selected to yield crystals when none might be observed in traditional initial screens. Besides, the screen includes heavy atoms for experimental phasing and small polyols to ensure the cryoprotection of crystals. The suitability of the resulting novel conditions is shown by the crystallization of a broad variety of protein samples and their efficiency is compared with commercially available conditions. International Union of Crystallography 2015-06-27 /pmc/articles/PMC4498703/ /pubmed/26144227 http://dx.doi.org/10.1107/S2053230X1500967X Text en © Gorrec 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Iccbm15 Gorrec, Fabrice The MORPHEUS II protein crystallization screen |
title | The MORPHEUS II protein crystallization screen |
title_full | The MORPHEUS II protein crystallization screen |
title_fullStr | The MORPHEUS II protein crystallization screen |
title_full_unstemmed | The MORPHEUS II protein crystallization screen |
title_short | The MORPHEUS II protein crystallization screen |
title_sort | morpheus ii protein crystallization screen |
topic | Iccbm15 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498703/ https://www.ncbi.nlm.nih.gov/pubmed/26144227 http://dx.doi.org/10.1107/S2053230X1500967X |
work_keys_str_mv | AT gorrecfabrice themorpheusiiproteincrystallizationscreen AT gorrecfabrice morpheusiiproteincrystallizationscreen |