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Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum

Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, es...

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Autores principales: Aibara, Shintaro, Valkov, Eugene, Lamers, Meindert H., Dimitrova, Lyudmila, Hurt, Ed, Stewart, Murray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498709/
https://www.ncbi.nlm.nih.gov/pubmed/26144233
http://dx.doi.org/10.1107/S2053230X15008766
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author Aibara, Shintaro
Valkov, Eugene
Lamers, Meindert H.
Dimitrova, Lyudmila
Hurt, Ed
Stewart, Murray
author_facet Aibara, Shintaro
Valkov, Eugene
Lamers, Meindert H.
Dimitrova, Lyudmila
Hurt, Ed
Stewart, Murray
author_sort Aibara, Shintaro
collection PubMed
description Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export.
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spelling pubmed-44987092015-07-28 Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum Aibara, Shintaro Valkov, Eugene Lamers, Meindert H. Dimitrova, Lyudmila Hurt, Ed Stewart, Murray Acta Crystallogr F Struct Biol Commun Research Communications Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export. International Union of Crystallography 2015-06-27 /pmc/articles/PMC4498709/ /pubmed/26144233 http://dx.doi.org/10.1107/S2053230X15008766 Text en © Aibara et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Communications
Aibara, Shintaro
Valkov, Eugene
Lamers, Meindert H.
Dimitrova, Lyudmila
Hurt, Ed
Stewart, Murray
Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title_full Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title_fullStr Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title_full_unstemmed Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title_short Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
title_sort structural characterization of the principal mrna-export factor mex67–mtr2 from chaetomium thermophilum
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498709/
https://www.ncbi.nlm.nih.gov/pubmed/26144233
http://dx.doi.org/10.1107/S2053230X15008766
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