Cargando…
Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, es...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498709/ https://www.ncbi.nlm.nih.gov/pubmed/26144233 http://dx.doi.org/10.1107/S2053230X15008766 |
_version_ | 1782380663169613824 |
---|---|
author | Aibara, Shintaro Valkov, Eugene Lamers, Meindert H. Dimitrova, Lyudmila Hurt, Ed Stewart, Murray |
author_facet | Aibara, Shintaro Valkov, Eugene Lamers, Meindert H. Dimitrova, Lyudmila Hurt, Ed Stewart, Murray |
author_sort | Aibara, Shintaro |
collection | PubMed |
description | Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export. |
format | Online Article Text |
id | pubmed-4498709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-44987092015-07-28 Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum Aibara, Shintaro Valkov, Eugene Lamers, Meindert H. Dimitrova, Lyudmila Hurt, Ed Stewart, Murray Acta Crystallogr F Struct Biol Commun Research Communications Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export. International Union of Crystallography 2015-06-27 /pmc/articles/PMC4498709/ /pubmed/26144233 http://dx.doi.org/10.1107/S2053230X15008766 Text en © Aibara et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Communications Aibara, Shintaro Valkov, Eugene Lamers, Meindert H. Dimitrova, Lyudmila Hurt, Ed Stewart, Murray Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum |
title | Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
|
title_full | Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
|
title_fullStr | Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
|
title_full_unstemmed | Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
|
title_short | Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum
|
title_sort | structural characterization of the principal mrna-export factor mex67–mtr2 from chaetomium thermophilum |
topic | Research Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498709/ https://www.ncbi.nlm.nih.gov/pubmed/26144233 http://dx.doi.org/10.1107/S2053230X15008766 |
work_keys_str_mv | AT aibarashintaro structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum AT valkoveugene structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum AT lamersmeinderth structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum AT dimitrovalyudmila structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum AT hurted structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum AT stewartmurray structuralcharacterizationoftheprincipalmrnaexportfactormex67mtr2fromchaetomiumthermophilum |