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Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea

Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that...

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Autores principales: Su, Liang-Chen, Deng, Bin, Liu, Shuai, Li, Li-Mei, Hu, Bo, Zhong, Yu-Ting, Li, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499716/
https://www.ncbi.nlm.nih.gov/pubmed/26217363
http://dx.doi.org/10.3389/fpls.2015.00512
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author Su, Liang-Chen
Deng, Bin
Liu, Shuai
Li, Li-Mei
Hu, Bo
Zhong, Yu-Ting
Li, Ling
author_facet Su, Liang-Chen
Deng, Bin
Liu, Shuai
Li, Li-Mei
Hu, Bo
Zhong, Yu-Ting
Li, Ling
author_sort Su, Liang-Chen
collection PubMed
description Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.
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spelling pubmed-44997162015-07-27 Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea Su, Liang-Chen Deng, Bin Liu, Shuai Li, Li-Mei Hu, Bo Zhong, Yu-Ting Li, Ling Front Plant Sci Plant Science Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA. Frontiers Media S.A. 2015-07-13 /pmc/articles/PMC4499716/ /pubmed/26217363 http://dx.doi.org/10.3389/fpls.2015.00512 Text en Copyright © 2015 Su, Deng, Liu, Li, Hu, Zhong and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Su, Liang-Chen
Deng, Bin
Liu, Shuai
Li, Li-Mei
Hu, Bo
Zhong, Yu-Ting
Li, Ling
Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title_full Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title_fullStr Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title_full_unstemmed Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title_short Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea
title_sort isolation and characterization of an osmotic stress and aba induced histone deacetylase in arachis hygogaea
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499716/
https://www.ncbi.nlm.nih.gov/pubmed/26217363
http://dx.doi.org/10.3389/fpls.2015.00512
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