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Radiofrequency treatment alters cancer cell phenotype

The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically diffe...

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Detalles Bibliográficos
Autores principales: Ware, Matthew J., Tinger, Sophia, Colbert, Kevin L., Corr, Stuart J., Rees, Paul, Koshkina, Nadezhda, Curley, Steven, Summers, H. D., Godin, Biana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499808/
https://www.ncbi.nlm.nih.gov/pubmed/26165830
http://dx.doi.org/10.1038/srep12083
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author Ware, Matthew J.
Tinger, Sophia
Colbert, Kevin L.
Corr, Stuart J.
Rees, Paul
Koshkina, Nadezhda
Curley, Steven
Summers, H. D.
Godin, Biana
author_facet Ware, Matthew J.
Tinger, Sophia
Colbert, Kevin L.
Corr, Stuart J.
Rees, Paul
Koshkina, Nadezhda
Curley, Steven
Summers, H. D.
Godin, Biana
author_sort Ware, Matthew J.
collection PubMed
description The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment.
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spelling pubmed-44998082015-07-17 Radiofrequency treatment alters cancer cell phenotype Ware, Matthew J. Tinger, Sophia Colbert, Kevin L. Corr, Stuart J. Rees, Paul Koshkina, Nadezhda Curley, Steven Summers, H. D. Godin, Biana Sci Rep Article The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment. Nature Publishing Group 2015-07-13 /pmc/articles/PMC4499808/ /pubmed/26165830 http://dx.doi.org/10.1038/srep12083 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ware, Matthew J.
Tinger, Sophia
Colbert, Kevin L.
Corr, Stuart J.
Rees, Paul
Koshkina, Nadezhda
Curley, Steven
Summers, H. D.
Godin, Biana
Radiofrequency treatment alters cancer cell phenotype
title Radiofrequency treatment alters cancer cell phenotype
title_full Radiofrequency treatment alters cancer cell phenotype
title_fullStr Radiofrequency treatment alters cancer cell phenotype
title_full_unstemmed Radiofrequency treatment alters cancer cell phenotype
title_short Radiofrequency treatment alters cancer cell phenotype
title_sort radiofrequency treatment alters cancer cell phenotype
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499808/
https://www.ncbi.nlm.nih.gov/pubmed/26165830
http://dx.doi.org/10.1038/srep12083
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