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TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes

Transforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes to study the expression and function of TACC3 during meiosis. TACC3 mRNA was detected in bovine oocytes du...

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Autores principales: Mahdipour, Mahdi, Leitoguinho, Ana Rita Canhoto, Zacarias Silva, Ricardo A., van Tol, Helena T. A., Stout, Tom A. E., Rodrigues, Gabriela, Roelen, Bernard A. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500572/
https://www.ncbi.nlm.nih.gov/pubmed/26168150
http://dx.doi.org/10.1371/journal.pone.0132591
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author Mahdipour, Mahdi
Leitoguinho, Ana Rita Canhoto
Zacarias Silva, Ricardo A.
van Tol, Helena T. A.
Stout, Tom A. E.
Rodrigues, Gabriela
Roelen, Bernard A. J.
author_facet Mahdipour, Mahdi
Leitoguinho, Ana Rita Canhoto
Zacarias Silva, Ricardo A.
van Tol, Helena T. A.
Stout, Tom A. E.
Rodrigues, Gabriela
Roelen, Bernard A. J.
author_sort Mahdipour, Mahdi
collection PubMed
description Transforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes to study the expression and function of TACC3 during meiosis. TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to demonstrate that TACC3 co-localized with tubulin in the metaphase I and II spindles. However, TACC3 was not detected at anaphase or telophase of the first meiotic division. Aurora A, which is known to phosphorylate and activate TACC3 in mitotic cells, showed a similar pattern of gene expression to that of TACC3 in meiotic oocytes and preimplantation embryos. Aurora A protein was however only very transiently associated to the meiotic spindle. Pharmaceutical inhibition of Aurora A activity inhibited TACC3 phosphorylation but did not prevent TACC3 appearance in the spindle. Inhibiting Aurora A activity did however lead to abnormal meiotic spindle formation and impaired maturation of bovine oocytes. Similar results were obtained by knock-down of TACC3 expression using siRNA injection. These results suggest that TACC3 is important for stabilizing the meiotic spindle, but phosphorylation of TACC3 by Aurora A is not required for its recruitment to the meiotic spindle although phosphorylation of TACC3 by other kinases cannot be excluded.
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spelling pubmed-45005722015-07-17 TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes Mahdipour, Mahdi Leitoguinho, Ana Rita Canhoto Zacarias Silva, Ricardo A. van Tol, Helena T. A. Stout, Tom A. E. Rodrigues, Gabriela Roelen, Bernard A. J. PLoS One Research Article Transforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes to study the expression and function of TACC3 during meiosis. TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to demonstrate that TACC3 co-localized with tubulin in the metaphase I and II spindles. However, TACC3 was not detected at anaphase or telophase of the first meiotic division. Aurora A, which is known to phosphorylate and activate TACC3 in mitotic cells, showed a similar pattern of gene expression to that of TACC3 in meiotic oocytes and preimplantation embryos. Aurora A protein was however only very transiently associated to the meiotic spindle. Pharmaceutical inhibition of Aurora A activity inhibited TACC3 phosphorylation but did not prevent TACC3 appearance in the spindle. Inhibiting Aurora A activity did however lead to abnormal meiotic spindle formation and impaired maturation of bovine oocytes. Similar results were obtained by knock-down of TACC3 expression using siRNA injection. These results suggest that TACC3 is important for stabilizing the meiotic spindle, but phosphorylation of TACC3 by Aurora A is not required for its recruitment to the meiotic spindle although phosphorylation of TACC3 by other kinases cannot be excluded. Public Library of Science 2015-07-13 /pmc/articles/PMC4500572/ /pubmed/26168150 http://dx.doi.org/10.1371/journal.pone.0132591 Text en © 2015 Mahdipour et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mahdipour, Mahdi
Leitoguinho, Ana Rita Canhoto
Zacarias Silva, Ricardo A.
van Tol, Helena T. A.
Stout, Tom A. E.
Rodrigues, Gabriela
Roelen, Bernard A. J.
TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title_full TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title_fullStr TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title_full_unstemmed TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title_short TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes
title_sort tacc3 is important for correct progression of meiosis in bovine oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500572/
https://www.ncbi.nlm.nih.gov/pubmed/26168150
http://dx.doi.org/10.1371/journal.pone.0132591
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