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In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139

In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of vari...

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Autores principales: Islam, Md. Saiful, Shahik, Shah Md., Sohel, Md., Patwary, Noman I. A., Hasan, Md. Anayet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Genome Organization 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500799/
https://www.ncbi.nlm.nih.gov/pubmed/26175663
http://dx.doi.org/10.5808/GI.2015.13.2.53
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author Islam, Md. Saiful
Shahik, Shah Md.
Sohel, Md.
Patwary, Noman I. A.
Hasan, Md. Anayet
author_facet Islam, Md. Saiful
Shahik, Shah Md.
Sohel, Md.
Patwary, Noman I. A.
Hasan, Md. Anayet
author_sort Islam, Md. Saiful
collection PubMed
description In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera.
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spelling pubmed-45007992015-07-14 In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139 Islam, Md. Saiful Shahik, Shah Md. Sohel, Md. Patwary, Noman I. A. Hasan, Md. Anayet Genomics Inform Original Article In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera. Korea Genome Organization 2015-06 2015-06-30 /pmc/articles/PMC4500799/ /pubmed/26175663 http://dx.doi.org/10.5808/GI.2015.13.2.53 Text en Copyright © 2015 by the Korea Genome Organization http://creativecommons.org/licenses/by-nc/3.0/ It is identical to the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Original Article
Islam, Md. Saiful
Shahik, Shah Md.
Sohel, Md.
Patwary, Noman I. A.
Hasan, Md. Anayet
In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title_full In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title_fullStr In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title_full_unstemmed In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title_short In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139
title_sort in silico structural and functional annotation of hypothetical proteins of vibrio cholerae o139
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500799/
https://www.ncbi.nlm.nih.gov/pubmed/26175663
http://dx.doi.org/10.5808/GI.2015.13.2.53
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