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P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes

P-bodies are cytoplasmic ribonucleoprotein granules involved in posttranscriptional regulation. DDX6 is a key component of their assembly in human cells. This DEAD-box RNA helicase is known to be associated with various complexes, including the decapping complex, the CPEB repression complex, RISC, a...

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Autores principales: Ayache, Jessica, Bénard, Marianne, Ernoult-Lange, Michèle, Minshall, Nicola, Standart, Nancy, Kress, Michel, Weil, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501357/
https://www.ncbi.nlm.nih.gov/pubmed/25995375
http://dx.doi.org/10.1091/mbc.E15-03-0136
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author Ayache, Jessica
Bénard, Marianne
Ernoult-Lange, Michèle
Minshall, Nicola
Standart, Nancy
Kress, Michel
Weil, Dominique
author_facet Ayache, Jessica
Bénard, Marianne
Ernoult-Lange, Michèle
Minshall, Nicola
Standart, Nancy
Kress, Michel
Weil, Dominique
author_sort Ayache, Jessica
collection PubMed
description P-bodies are cytoplasmic ribonucleoprotein granules involved in posttranscriptional regulation. DDX6 is a key component of their assembly in human cells. This DEAD-box RNA helicase is known to be associated with various complexes, including the decapping complex, the CPEB repression complex, RISC, and the CCR4/NOT complex. To understand which DDX6 complexes are required for P-body assembly, we analyzed the DDX6 interactome using the tandem-affinity purification methodology coupled to mass spectrometry. Three complexes were prominent: the decapping complex, a CPEB-like complex, and an Ataxin2/Ataxin2L complex. The exon junction complex was also found, suggesting DDX6 binding to newly exported mRNAs. Finally, some DDX6 was associated with polysomes, as previously reported in yeast. Despite its high enrichment in P-bodies, most DDX6 is localized out of P-bodies. Of the three complexes, only the decapping and CPEB-like complexes were recruited into P-bodies. Investigation of P-body assembly in various conditions allowed us to distinguish required proteins from those that are dispensable or participate only in specific conditions. Three proteins were required in all tested conditions: DDX6, 4E-T, and LSM14A. These results reveal the variety of pathways of P-body assembly, which all nevertheless share three key factors connecting P-body assembly to repression.
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spelling pubmed-45013572015-09-30 P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes Ayache, Jessica Bénard, Marianne Ernoult-Lange, Michèle Minshall, Nicola Standart, Nancy Kress, Michel Weil, Dominique Mol Biol Cell Articles P-bodies are cytoplasmic ribonucleoprotein granules involved in posttranscriptional regulation. DDX6 is a key component of their assembly in human cells. This DEAD-box RNA helicase is known to be associated with various complexes, including the decapping complex, the CPEB repression complex, RISC, and the CCR4/NOT complex. To understand which DDX6 complexes are required for P-body assembly, we analyzed the DDX6 interactome using the tandem-affinity purification methodology coupled to mass spectrometry. Three complexes were prominent: the decapping complex, a CPEB-like complex, and an Ataxin2/Ataxin2L complex. The exon junction complex was also found, suggesting DDX6 binding to newly exported mRNAs. Finally, some DDX6 was associated with polysomes, as previously reported in yeast. Despite its high enrichment in P-bodies, most DDX6 is localized out of P-bodies. Of the three complexes, only the decapping and CPEB-like complexes were recruited into P-bodies. Investigation of P-body assembly in various conditions allowed us to distinguish required proteins from those that are dispensable or participate only in specific conditions. Three proteins were required in all tested conditions: DDX6, 4E-T, and LSM14A. These results reveal the variety of pathways of P-body assembly, which all nevertheless share three key factors connecting P-body assembly to repression. The American Society for Cell Biology 2015-07-15 /pmc/articles/PMC4501357/ /pubmed/25995375 http://dx.doi.org/10.1091/mbc.E15-03-0136 Text en © 2015 Ayache, Bénard, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Ayache, Jessica
Bénard, Marianne
Ernoult-Lange, Michèle
Minshall, Nicola
Standart, Nancy
Kress, Michel
Weil, Dominique
P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title_full P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title_fullStr P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title_full_unstemmed P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title_short P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
title_sort p-body assembly requires ddx6 repression complexes rather than decay or ataxin2/2l complexes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501357/
https://www.ncbi.nlm.nih.gov/pubmed/25995375
http://dx.doi.org/10.1091/mbc.E15-03-0136
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