A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines

We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-...

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Autores principales: Bickersmith, Sara A, Lainhart, William, Moreno, Marta, Chu, Virginia M, Vinetz, Joseph M, Conn, Jan E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501424/
https://www.ncbi.nlm.nih.gov/pubmed/26061150
http://dx.doi.org/10.1590/0074-02760150031
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author Bickersmith, Sara A
Lainhart, William
Moreno, Marta
Chu, Virginia M
Vinetz, Joseph M
Conn, Jan E
author_facet Bickersmith, Sara A
Lainhart, William
Moreno, Marta
Chu, Virginia M
Vinetz, Joseph M
Conn, Jan E
author_sort Bickersmith, Sara A
collection PubMed
description We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.
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spelling pubmed-45014242015-07-16 A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines Bickersmith, Sara A Lainhart, William Moreno, Marta Chu, Virginia M Vinetz, Joseph M Conn, Jan E Mem Inst Oswaldo Cruz Technical Note We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible. Instituto Oswaldo Cruz, Ministério da Saúde 2015-06 /pmc/articles/PMC4501424/ /pubmed/26061150 http://dx.doi.org/10.1590/0074-02760150031 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Bickersmith, Sara A
Lainhart, William
Moreno, Marta
Chu, Virginia M
Vinetz, Joseph M
Conn, Jan E
A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title_full A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title_fullStr A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title_full_unstemmed A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title_short A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
title_sort sensitive, specific and reproducible real-time polymerase chain reaction method for detection of plasmodium vivax and plasmodium falciparum infection in field-collected anophelines
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501424/
https://www.ncbi.nlm.nih.gov/pubmed/26061150
http://dx.doi.org/10.1590/0074-02760150031
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