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A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines

We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-...

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Detalles Bibliográficos
Autores principales:	Bickersmith, Sara A, Lainhart, William, Moreno, Marta, Chu, Virginia M, Vinetz, Joseph M, Conn, Jan E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501424/ https://www.ncbi.nlm.nih.gov/pubmed/26061150 http://dx.doi.org/10.1590/0074-02760150031

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