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Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells

CD44 is a prominent activation marker which distinguishes memory and effector T cells from their naïve counterparts. It also plays a role in early T cell signaling events as it is bound to the lymphocyte-specific protein kinase and thereby enhances T cell receptor signalling. Here, we investigated w...

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Autores principales: Schumann, Julia, Stanko, Katarina, Schliesser, Ulrike, Appelt, Christine, Sawitzki, Birgit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501817/
https://www.ncbi.nlm.nih.gov/pubmed/26172046
http://dx.doi.org/10.1371/journal.pone.0132479
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author Schumann, Julia
Stanko, Katarina
Schliesser, Ulrike
Appelt, Christine
Sawitzki, Birgit
author_facet Schumann, Julia
Stanko, Katarina
Schliesser, Ulrike
Appelt, Christine
Sawitzki, Birgit
author_sort Schumann, Julia
collection PubMed
description CD44 is a prominent activation marker which distinguishes memory and effector T cells from their naïve counterparts. It also plays a role in early T cell signaling events as it is bound to the lymphocyte-specific protein kinase and thereby enhances T cell receptor signalling. Here, we investigated whether IFN-γ and IL-17 producing T helper cells differ in their CD44 expression and their dependence of CD44 for differentiation. Stimulation of CD4(+) T cells with allogeneic dendritic cells resulted in the formation of three distinguishable populations: CD44(+), CD44(++) and CD44(+++). In vitro and in vivo generated allo-reactive IL-17 producing T helper cells were mainly CD44(+++) as compared to IFN-γ(+) T helper cells, which were CD44(++). This effect was enhanced under polarizing conditions. T helper 17 polarization led to a shift towards the CD44(+++) population, whereas T helper 1 polarization diminished this population. Furthermore, blocking CD44 decreased IL-17 secretion, while IFN-γ was barely affected. Titration experiments revealed that low T cell receptor and CD28 stimulation supported T helper 17 rather than T helper 1 development. Under these conditions CD44 could act as a co-stimulatory molecule and replace CD28. Indeed, rested CD44(+++)CD4(+) T cells contained already more total and especially phosphorylated zeta-chain-associated protein kinase 70 as compared to CD44(++) cells. Our results support the notion, that CD44 enhances T cell receptor signaling strength by delivering lymphocyte-specific protein kinase, which is required for induction of IL-17 producing T helper cells.
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spelling pubmed-45018172015-07-17 Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells Schumann, Julia Stanko, Katarina Schliesser, Ulrike Appelt, Christine Sawitzki, Birgit PLoS One Research Article CD44 is a prominent activation marker which distinguishes memory and effector T cells from their naïve counterparts. It also plays a role in early T cell signaling events as it is bound to the lymphocyte-specific protein kinase and thereby enhances T cell receptor signalling. Here, we investigated whether IFN-γ and IL-17 producing T helper cells differ in their CD44 expression and their dependence of CD44 for differentiation. Stimulation of CD4(+) T cells with allogeneic dendritic cells resulted in the formation of three distinguishable populations: CD44(+), CD44(++) and CD44(+++). In vitro and in vivo generated allo-reactive IL-17 producing T helper cells were mainly CD44(+++) as compared to IFN-γ(+) T helper cells, which were CD44(++). This effect was enhanced under polarizing conditions. T helper 17 polarization led to a shift towards the CD44(+++) population, whereas T helper 1 polarization diminished this population. Furthermore, blocking CD44 decreased IL-17 secretion, while IFN-γ was barely affected. Titration experiments revealed that low T cell receptor and CD28 stimulation supported T helper 17 rather than T helper 1 development. Under these conditions CD44 could act as a co-stimulatory molecule and replace CD28. Indeed, rested CD44(+++)CD4(+) T cells contained already more total and especially phosphorylated zeta-chain-associated protein kinase 70 as compared to CD44(++) cells. Our results support the notion, that CD44 enhances T cell receptor signaling strength by delivering lymphocyte-specific protein kinase, which is required for induction of IL-17 producing T helper cells. Public Library of Science 2015-07-14 /pmc/articles/PMC4501817/ /pubmed/26172046 http://dx.doi.org/10.1371/journal.pone.0132479 Text en © 2015 Schumann et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schumann, Julia
Stanko, Katarina
Schliesser, Ulrike
Appelt, Christine
Sawitzki, Birgit
Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title_full Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title_fullStr Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title_full_unstemmed Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title_short Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells
title_sort differences in cd44 surface expression levels and function discriminates il-17 and ifn-γ producing helper t cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501817/
https://www.ncbi.nlm.nih.gov/pubmed/26172046
http://dx.doi.org/10.1371/journal.pone.0132479
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