Cargando…

Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research

The Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR associated (CRISPR/Cas) technology allows rapid, site-specific genome modification in a wide variety of organisms . Proof-of-principle studies in Drosophila melanogaster have used various CRISPR/Cas tools and experimental designs, l...

Descripción completa

Detalles Bibliográficos
Autores principales:	Port, Fillip, Muschalik, Nadine, Bullock, Simon L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2015
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502383/ https://www.ncbi.nlm.nih.gov/pubmed/25999583 http://dx.doi.org/10.1534/g3.115.019083

Ejemplares similares

A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila
por: Doll, Roman M., et al.
Publicado: (2023)

Conditional CRISPR-Cas Genome Editing in Drosophila to Generate Intestinal Tumors
por: Bahuguna, Shivohum, et al.
Publicado: (2021)

RNA Interference (RNAi) Screening in Drosophila
por: Heigwer, Florian, et al.
Publicado: (2018)

Robust Wnt signaling is maintained by a Wg protein gradient and Fz2 receptor activity in the developing Drosophila wing
por: Chaudhary, Varun, et al.
Publicado: (2019)

Multiplexed conditional genome editing with Cas12a in Drosophila
por: Port, Fillip, et al.
Publicado: (2020)

Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy
por: Kane, Nanci S., et al.
Publicado: (2016)

CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila
por: Xue, Zhaoyu, et al.
Publicado: (2014)

Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogaster
por: Verma, Shreekant, et al.
Publicado: (2021)

Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae
por: Roggenkamp, Emily, et al.
Publicado: (2018)

Neurofibromin Loss of Function Drives Excessive Grooming in Drosophila
por: King, Lanikea B., et al.
Publicado: (2016)

A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
por: Port, Fillip, et al.
Publicado: (2020)

Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila
por: Gokcezade, Joseph, et al.
Publicado: (2014)

Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion
por: Ge, Daniel Tianfang, et al.
Publicado: (2016)

Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila
por: Gratz, Scott J., et al.
Publicado: (2014)

Generation of Drosophila attP containing cell lines using CRISPR-Cas9
por: Mariyappa, Daniel, et al.
Publicado: (2021)

The Interplay of Temperature and Genotype on Patterns of Alternative Splicing in Drosophila melanogaster
por: Jakšić, Ana Marija, et al.
Publicado: (2016)

Can a Population Targeted by a CRISPR-Based Homing Gene Drive Be Rescued?
por: Rode, Nicolas O., et al.
Publicado: (2020)

Combinatorial Effects of Transposable Elements on Gene Expression and Phenotypic Robustness in Drosophila melanogaster Development
por: Clemmons, Alexa W., et al.
Publicado: (2013)

Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans
por: Stevenson, Zachary C., et al.
Publicado: (2020)

A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila
por: Zhang, Xu, et al.
Publicado: (2014)

Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster
por: Loehlin, David W, et al.
Publicado: (2023)

How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics
por: Roote, John, et al.
Publicado: (2013)

Non-Mendelian Dominant Maternal Effects Caused by CRISPR/Cas9 Transgenic Components in Drosophila melanogaster
por: Lin, Chun-Chieh, et al.
Publicado: (2016)

ovo(D) Co-selection: A Method for Enriching CRISPR/Cas9-Edited Alleles in Drosophila
por: Ewen-Campen, Ben, et al.
Publicado: (2018)

Sequence Analysis of the Segmental Duplication Responsible for Paris Sex-Ratio Drive in Drosophila simulans
por: Fouvry, Lucie, et al.
Publicado: (2011)

Checkpoint activation drives global gene expression changes in Drosophila nuclear lamina mutants
por: Kitzman, Samuel Cole, et al.
Publicado: (2021)

CRISPR-Cas-Induced Mutants Identify a Requirement for dSTIM in Larval Dopaminergic Cells of Drosophila melanogaster
por: Pathak, Trayambak, et al.
Publicado: (2017)

Tools for Targeted Genome Engineering of Established Drosophila Cell Lines
por: Cherbas, Lucy, et al.
Publicado: (2015)

In vivo characterization of Drosophila golgins reveals redundancy and plasticity of vesicle capture at the Golgi apparatus
por: Park, Sung Yun, et al.
Publicado: (2022)

Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase
por: Andjelković, Ana, et al.
Publicado: (2016)

Age-dependent impairment of disease tolerance is associated with a robust transcriptional response following RNA virus infection in Drosophila
por: Sheffield, Lakbira, et al.
Publicado: (2021)

Examination of Endogenous Rotund Expression and Function in Developing Drosophila Olfactory System Using CRISPR-Cas9–Mediated Protein Tagging
por: Li, Qingyun, et al.
Publicado: (2015)

Genetic Approaches for Controlling CRISPR-based Autonomous Homing Gene Drives
por: Chennuri, Pratima R., et al.
Publicado: (2022)

A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues
por: Huynh, Nhan, et al.
Publicado: (2018)

Deep learning-based robust positioning for all-weather autonomous driving
por: Almalioglu, Yasin, et al.
Publicado: (2022)

CRISpy-Pop: A Web Tool for Designing CRISPR/Cas9-Driven Genetic Modifications in Diverse Populations
por: Stoneman, Hayley R., et al.
Publicado: (2020)

Improving Transgenesis Efficiency and CRISPR-Associated Tools Through Codon Optimization and Native Intron Addition in Pristionchus Nematodes
por: Han, Ziduan, et al.
Publicado: (2020)

Potential risk assessment for safe driving of autonomous vehicles under occluded vision
por: Wang, Denggui, et al.
Publicado: (2022)

Safe, Efficient, and Comfortable Autonomous Driving Based on Cooperative Vehicle Infrastructure System
por: Chen, Jing, et al.
Publicado: (2023)

Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling
por: Reid, William, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ie8r2e5sem00p5mbe5egg7gof9