Cargando…

Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study

BACKGROUND: Transcatheter pulmonary valve replacement is currently performed in clinical trials, but is limited by the use of glutaraldehyde-treated bioprostheses. This feasibility study was performed to evaluate delivery-related tissue distortion during implantation of tissue-engineered (TE) heart...

Descripción completa

Detalles Bibliográficos
Autores principales: Schlegel, Franziska, Salameh, Aida, Oelmann, Katja, Halling, Michelle, Dhein, Stefan, Mohr, Friedrich W., Dohmen, Pascal M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502544/
https://www.ncbi.nlm.nih.gov/pubmed/26104851
http://dx.doi.org/10.12659/MSMBR.894838
_version_ 1782381227480711168
author Schlegel, Franziska
Salameh, Aida
Oelmann, Katja
Halling, Michelle
Dhein, Stefan
Mohr, Friedrich W.
Dohmen, Pascal M.
author_facet Schlegel, Franziska
Salameh, Aida
Oelmann, Katja
Halling, Michelle
Dhein, Stefan
Mohr, Friedrich W.
Dohmen, Pascal M.
author_sort Schlegel, Franziska
collection PubMed
description BACKGROUND: Transcatheter pulmonary valve replacement is currently performed in clinical trials, but is limited by the use of glutaraldehyde-treated bioprostheses. This feasibility study was performed to evaluate delivery-related tissue distortion during implantation of tissue-engineered (TE) heart valves. MATERIAL/METHODS: The injectable TE heart valve was mounted on a self-expanding nitinol stent (n=7) and delivered into the pulmonary position in 7 pigs, (weight 26 to 31 kg), performing a sternotomy or limited lateral thoracotomy. Prior to implantation, the injectable TE heart valves were crimped and inserted into an applicator. Positioning of the implants was guided by fluoroscopy, and after careful deployment, angiographic examination was performed to evaluate the correct delivered position. Hemodynamic measurements were performed by epicardial echocardiography. Finally, the animals were sacrificed and the injectable TE heart valves were inspected by gross examination and histological examination. RESULTS: Orthotopic deliveries of the injectable TE heart valves were all successful performed, expect in 1 where the valve migrated due to a discrepancy between pulmonary valve annulus size and injectable TE valve size. Angiographic evaluation (n=6) showed normal valve function, supported by epicardial echocardiography in which no increased flow velocity was measured, neither trans- nor paravalvular regurgitation. Histological evaluation demonstrated absence of tissue damage from the delivery process. CONCLUSIONS: Transcatheter implantation of an injectable TE heart valve seems to be possible without tissue distortion due to the delivery system.
format Online
Article
Text
id pubmed-4502544
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-45025442015-07-17 Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study Schlegel, Franziska Salameh, Aida Oelmann, Katja Halling, Michelle Dhein, Stefan Mohr, Friedrich W. Dohmen, Pascal M. Med Sci Monit Basic Res Animal Studies BACKGROUND: Transcatheter pulmonary valve replacement is currently performed in clinical trials, but is limited by the use of glutaraldehyde-treated bioprostheses. This feasibility study was performed to evaluate delivery-related tissue distortion during implantation of tissue-engineered (TE) heart valves. MATERIAL/METHODS: The injectable TE heart valve was mounted on a self-expanding nitinol stent (n=7) and delivered into the pulmonary position in 7 pigs, (weight 26 to 31 kg), performing a sternotomy or limited lateral thoracotomy. Prior to implantation, the injectable TE heart valves were crimped and inserted into an applicator. Positioning of the implants was guided by fluoroscopy, and after careful deployment, angiographic examination was performed to evaluate the correct delivered position. Hemodynamic measurements were performed by epicardial echocardiography. Finally, the animals were sacrificed and the injectable TE heart valves were inspected by gross examination and histological examination. RESULTS: Orthotopic deliveries of the injectable TE heart valves were all successful performed, expect in 1 where the valve migrated due to a discrepancy between pulmonary valve annulus size and injectable TE valve size. Angiographic evaluation (n=6) showed normal valve function, supported by epicardial echocardiography in which no increased flow velocity was measured, neither trans- nor paravalvular regurgitation. Histological evaluation demonstrated absence of tissue damage from the delivery process. CONCLUSIONS: Transcatheter implantation of an injectable TE heart valve seems to be possible without tissue distortion due to the delivery system. International Scientific Literature, Inc. 2015-06-24 /pmc/articles/PMC4502544/ /pubmed/26104851 http://dx.doi.org/10.12659/MSMBR.894838 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
spellingShingle Animal Studies
Schlegel, Franziska
Salameh, Aida
Oelmann, Katja
Halling, Michelle
Dhein, Stefan
Mohr, Friedrich W.
Dohmen, Pascal M.
Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title_full Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title_fullStr Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title_full_unstemmed Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title_short Injectable Tissue-Engineered Pulmonary Heart Valve Implantation Into the Pig Model: A Feasibility Study
title_sort injectable tissue-engineered pulmonary heart valve implantation into the pig model: a feasibility study
topic Animal Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502544/
https://www.ncbi.nlm.nih.gov/pubmed/26104851
http://dx.doi.org/10.12659/MSMBR.894838
work_keys_str_mv AT schlegelfranziska injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT salamehaida injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT oelmannkatja injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT hallingmichelle injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT dheinstefan injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT mohrfriedrichw injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy
AT dohmenpascalm injectabletissueengineeredpulmonaryheartvalveimplantationintothepigmodelafeasibilitystudy