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Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease

BACKGROUND: The disorder of lipid metabolism and genetic predisposition are major risk factors for coronary artery disease (CAD). Variants in the apolipoprotein A1 (APOA1) gene play an important role in the regulation of lipids. The objective of the present study was to investigate the effect of two...

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Autores principales: Liao, BiHong, Cheng, KeQi, Dong, ShaoHong, Liu, HuaDong, Xu, ZhengLei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502599/
https://www.ncbi.nlm.nih.gov/pubmed/26173491
http://dx.doi.org/10.1186/s13000-015-0328-7
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author Liao, BiHong
Cheng, KeQi
Dong, ShaoHong
Liu, HuaDong
Xu, ZhengLei
author_facet Liao, BiHong
Cheng, KeQi
Dong, ShaoHong
Liu, HuaDong
Xu, ZhengLei
author_sort Liao, BiHong
collection PubMed
description BACKGROUND: The disorder of lipid metabolism and genetic predisposition are major risk factors for coronary artery disease (CAD). Variants in the apolipoprotein A1 (APOA1) gene play an important role in the regulation of lipids. The objective of the present study was to investigate the effect of two polymorphisms (-75 G/A and +83 C/T) of APOA1 on lipid profiles and the risk of CAD. METHODS: A total number of 300 subjects with CAD and 300 age and sex matched healthy controls were enrolled for the study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing. RESULTS: The frequencies of APOA1 -75 AA genotype [odds ratio (OR) =0.50, 95 % confidence interval (CI) = 0.28, 0.88; P = 0.02] and APOA1 -75 A allele (OR =0.76, 95 % CI = 0.59, 0.98; P = 0.04) were significantly lower in CAD than in controls. The APOA1 -75 A allele was significantly associated with increasing serum concentrations of ApoA1 and high-density lipoprotein cholesterol (HDL-C) (P < 0.001). CONCLUSIONS: The individuals with the APOA1 -75 A allele were likely to have a lower risk of CAD as a result of its effect on higher serum concentrations of ApoA1 and HDL-C.
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spelling pubmed-45025992015-07-16 Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease Liao, BiHong Cheng, KeQi Dong, ShaoHong Liu, HuaDong Xu, ZhengLei Diagn Pathol Research BACKGROUND: The disorder of lipid metabolism and genetic predisposition are major risk factors for coronary artery disease (CAD). Variants in the apolipoprotein A1 (APOA1) gene play an important role in the regulation of lipids. The objective of the present study was to investigate the effect of two polymorphisms (-75 G/A and +83 C/T) of APOA1 on lipid profiles and the risk of CAD. METHODS: A total number of 300 subjects with CAD and 300 age and sex matched healthy controls were enrolled for the study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing. RESULTS: The frequencies of APOA1 -75 AA genotype [odds ratio (OR) =0.50, 95 % confidence interval (CI) = 0.28, 0.88; P = 0.02] and APOA1 -75 A allele (OR =0.76, 95 % CI = 0.59, 0.98; P = 0.04) were significantly lower in CAD than in controls. The APOA1 -75 A allele was significantly associated with increasing serum concentrations of ApoA1 and high-density lipoprotein cholesterol (HDL-C) (P < 0.001). CONCLUSIONS: The individuals with the APOA1 -75 A allele were likely to have a lower risk of CAD as a result of its effect on higher serum concentrations of ApoA1 and HDL-C. BioMed Central 2015-07-16 /pmc/articles/PMC4502599/ /pubmed/26173491 http://dx.doi.org/10.1186/s13000-015-0328-7 Text en © Liao et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liao, BiHong
Cheng, KeQi
Dong, ShaoHong
Liu, HuaDong
Xu, ZhengLei
Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title_full Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title_fullStr Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title_full_unstemmed Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title_short Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
title_sort effect of apolipoprotein a1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502599/
https://www.ncbi.nlm.nih.gov/pubmed/26173491
http://dx.doi.org/10.1186/s13000-015-0328-7
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