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A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications
The lack of a rapid and quantitative autophagy assay has substantially hindered the development and implementation of autophagy-targeting therapies for a variety of human diseases. To address this critical issue, we developed a novel autophagy assay using the newly developed Cyto-ID fluorescence dye...
Autores principales: | Guo, Sujuan, Liang, Yanping, Murphy, Susan F, Huang, Angela, Shen, Haihong, Kelly, Deborah F, Sobrado, Pablo, Sheng, Zhi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502761/ https://www.ncbi.nlm.nih.gov/pubmed/25714620 http://dx.doi.org/10.1080/15548627.2015.1017181 |
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