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CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis
Background and Aims. Inflammatory mediators that cross-talk in different metabolically active organs are thought to play a crucial role in the pathogenesis of Nonalcoholic Steatohepatitis (NASH). This study was aimed at investigating the CD4+RORγt+ T-helper cells and their counterpart, the CD4+CD25+...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503578/ https://www.ncbi.nlm.nih.gov/pubmed/26229237 http://dx.doi.org/10.1155/2015/239623 |
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author | Vonghia, Luisa Ruyssers, Nathalie Schrijvers, Dorien Pelckmans, Paul Michielsen, Peter De Clerck, Luc Ramon, Albert Jirillo, Emilio Ebo, Didier De Winter, Benedicte Bridts, Chris Francque, Sven |
author_facet | Vonghia, Luisa Ruyssers, Nathalie Schrijvers, Dorien Pelckmans, Paul Michielsen, Peter De Clerck, Luc Ramon, Albert Jirillo, Emilio Ebo, Didier De Winter, Benedicte Bridts, Chris Francque, Sven |
author_sort | Vonghia, Luisa |
collection | PubMed |
description | Background and Aims. Inflammatory mediators that cross-talk in different metabolically active organs are thought to play a crucial role in the pathogenesis of Nonalcoholic Steatohepatitis (NASH). This study was aimed at investigating the CD4+RORγt+ T-helper cells and their counterpart, the CD4+CD25+FOXP3+ regulatory T cells in the liver, subcutaneous adipose tissue (SAT), and abdominal adipose tissue (AAT) in a high fat diet (HFD) mouse model. Methods. C57BL6 mice were fed a HFD or a normal diet (ND). Liver enzymes, metabolic parameters, and liver histology were assessed. The expression of CD4+RORγt+ cells and regulatory T cells in different organs (blood, liver, AAT, and SAT) were analyzed by flow cytometry. Cytokine and adipokine tissue expression were studied by RT-PCR. Results. Mice fed a HFD developed NASH and metabolic alterations compared to normal diet. CD4+RORγt++ cells were significantly increased in the liver and the AAT while an increase of regulatory T cells was observed in the SAT of mice fed HFD compared to ND. Inflammatory cytokines were also upregulated. Conclusions. CD4+RORγt++ cells and regulatory T cells are altered in NASH with a site-specific pattern and correlate with the severity of the disease. These site-specific differences are associated with increased cytokine expression. |
format | Online Article Text |
id | pubmed-4503578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45035782015-07-30 CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis Vonghia, Luisa Ruyssers, Nathalie Schrijvers, Dorien Pelckmans, Paul Michielsen, Peter De Clerck, Luc Ramon, Albert Jirillo, Emilio Ebo, Didier De Winter, Benedicte Bridts, Chris Francque, Sven Mediators Inflamm Research Article Background and Aims. Inflammatory mediators that cross-talk in different metabolically active organs are thought to play a crucial role in the pathogenesis of Nonalcoholic Steatohepatitis (NASH). This study was aimed at investigating the CD4+RORγt+ T-helper cells and their counterpart, the CD4+CD25+FOXP3+ regulatory T cells in the liver, subcutaneous adipose tissue (SAT), and abdominal adipose tissue (AAT) in a high fat diet (HFD) mouse model. Methods. C57BL6 mice were fed a HFD or a normal diet (ND). Liver enzymes, metabolic parameters, and liver histology were assessed. The expression of CD4+RORγt+ cells and regulatory T cells in different organs (blood, liver, AAT, and SAT) were analyzed by flow cytometry. Cytokine and adipokine tissue expression were studied by RT-PCR. Results. Mice fed a HFD developed NASH and metabolic alterations compared to normal diet. CD4+RORγt++ cells were significantly increased in the liver and the AAT while an increase of regulatory T cells was observed in the SAT of mice fed HFD compared to ND. Inflammatory cytokines were also upregulated. Conclusions. CD4+RORγt++ cells and regulatory T cells are altered in NASH with a site-specific pattern and correlate with the severity of the disease. These site-specific differences are associated with increased cytokine expression. Hindawi Publishing Corporation 2015 2015-07-01 /pmc/articles/PMC4503578/ /pubmed/26229237 http://dx.doi.org/10.1155/2015/239623 Text en Copyright © 2015 Luisa Vonghia et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Vonghia, Luisa Ruyssers, Nathalie Schrijvers, Dorien Pelckmans, Paul Michielsen, Peter De Clerck, Luc Ramon, Albert Jirillo, Emilio Ebo, Didier De Winter, Benedicte Bridts, Chris Francque, Sven CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title | CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title_full | CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title_fullStr | CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title_full_unstemmed | CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title_short | CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis |
title_sort | cd4+rorγt++ and tregs in a mouse model of diet-induced nonalcoholic steatohepatitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503578/ https://www.ncbi.nlm.nih.gov/pubmed/26229237 http://dx.doi.org/10.1155/2015/239623 |
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