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Neuroprotective Activity of Coptisine from Coptis chinensis (Franch)
Coptis chinensis rhizomes (CR) are one important ingredient of traditional Chinese herbal formulas such as San-Huang-Xie-Xin-Tang which is used for treatment of cardiovascular and neurodegenerative diseases. Recent studies suggest that the extract of CR might be a potential therapeutic agent for ame...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503580/ https://www.ncbi.nlm.nih.gov/pubmed/26229546 http://dx.doi.org/10.1155/2015/827308 |
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author | Friedemann, Thomas Schumacher, Udo Tao, Yi Leung, Alexander Kai-Man Schröder, Sven |
author_facet | Friedemann, Thomas Schumacher, Udo Tao, Yi Leung, Alexander Kai-Man Schröder, Sven |
author_sort | Friedemann, Thomas |
collection | PubMed |
description | Coptis chinensis rhizomes (CR) are one important ingredient of traditional Chinese herbal formulas such as San-Huang-Xie-Xin-Tang which is used for treatment of cardiovascular and neurodegenerative diseases. Recent studies suggest that the extract of CR might be a potential therapeutic agent for amelioration of neurological disorders associated with oxidative stress. In the present study we aimed at revealing the main active compound(s) of the CR extract and at investigating the mechanism of action. Four main alkaloids of the CR extract (berberine, coptisine, jatrorrhizine, and palmatine) were selected for this study. Results showed that out of those alkaloids only pretreatment with coptisine significantly attenuated tert-butylhydroperoxide induced reduction of cell viability, increased rate of apoptosis, and declined mitochondrial membrane potential. Elisa assay and quantitative real-time PCR analyses revealed that thioredoxin-interacting protein (TXNIP) gene expression was downregulated by coptisine, which could explain the neuroprotective effect, hypothetically, by strengthening the thioredoxin defense system against oxidative stress and attenuation of apoptosis signal-regulating kinase (Ask1) mediated apoptotic signaling. A comparison between coptisine and CR extract identified coptisine as the main single component responsible for the neuroprotective effect. Based on the results the CR extract and coptisine are promising candidate agents for prevention or improvement of diabetic neuropathy and neurodegenerative disorders. |
format | Online Article Text |
id | pubmed-4503580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45035802015-07-30 Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) Friedemann, Thomas Schumacher, Udo Tao, Yi Leung, Alexander Kai-Man Schröder, Sven Evid Based Complement Alternat Med Research Article Coptis chinensis rhizomes (CR) are one important ingredient of traditional Chinese herbal formulas such as San-Huang-Xie-Xin-Tang which is used for treatment of cardiovascular and neurodegenerative diseases. Recent studies suggest that the extract of CR might be a potential therapeutic agent for amelioration of neurological disorders associated with oxidative stress. In the present study we aimed at revealing the main active compound(s) of the CR extract and at investigating the mechanism of action. Four main alkaloids of the CR extract (berberine, coptisine, jatrorrhizine, and palmatine) were selected for this study. Results showed that out of those alkaloids only pretreatment with coptisine significantly attenuated tert-butylhydroperoxide induced reduction of cell viability, increased rate of apoptosis, and declined mitochondrial membrane potential. Elisa assay and quantitative real-time PCR analyses revealed that thioredoxin-interacting protein (TXNIP) gene expression was downregulated by coptisine, which could explain the neuroprotective effect, hypothetically, by strengthening the thioredoxin defense system against oxidative stress and attenuation of apoptosis signal-regulating kinase (Ask1) mediated apoptotic signaling. A comparison between coptisine and CR extract identified coptisine as the main single component responsible for the neuroprotective effect. Based on the results the CR extract and coptisine are promising candidate agents for prevention or improvement of diabetic neuropathy and neurodegenerative disorders. Hindawi Publishing Corporation 2015 2015-07-02 /pmc/articles/PMC4503580/ /pubmed/26229546 http://dx.doi.org/10.1155/2015/827308 Text en Copyright © 2015 Thomas Friedemann et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Friedemann, Thomas Schumacher, Udo Tao, Yi Leung, Alexander Kai-Man Schröder, Sven Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title | Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title_full | Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title_fullStr | Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title_full_unstemmed | Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title_short | Neuroprotective Activity of Coptisine from Coptis chinensis (Franch) |
title_sort | neuroprotective activity of coptisine from coptis chinensis (franch) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503580/ https://www.ncbi.nlm.nih.gov/pubmed/26229546 http://dx.doi.org/10.1155/2015/827308 |
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