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Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer

The purpose of this study was to develop quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the analysis of proteins involved in metastasis of breast cancer for diagnosis and determining disease prognosis, as well as to further our understand of metastatic mechanisms....

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Autores principales: Goto, Rieko, Nakamura, Yasushi, Takami, Tomonori, Sanke, Tokio, Tozuka, Zenzaburo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503764/
https://www.ncbi.nlm.nih.gov/pubmed/26176947
http://dx.doi.org/10.1371/journal.pone.0130760
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author Goto, Rieko
Nakamura, Yasushi
Takami, Tomonori
Sanke, Tokio
Tozuka, Zenzaburo
author_facet Goto, Rieko
Nakamura, Yasushi
Takami, Tomonori
Sanke, Tokio
Tozuka, Zenzaburo
author_sort Goto, Rieko
collection PubMed
description The purpose of this study was to develop quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the analysis of proteins involved in metastasis of breast cancer for diagnosis and determining disease prognosis, as well as to further our understand of metastatic mechanisms. We have previously demonstrated that the protein type XIV collagen may be specifically expressed in metastatic tissues by two dimensional LC-MS/MS. In this study, we developed quantitative LC-MS/MS methods for type XIV collagen. Type XIV collagen was quantified by analyzing 2 peptides generated by digesting type XIV collagen using stable isotope-labeled peptides. The individual concentrations were equivalent between 2 different peptides of type XIV collagen by evaluation of imprecise transitions and using the best transition for the peptide concentration. The results indicated that type XIV collagen is highly expressed in metastatic tissues of patients with massive lymph node involvement compared to non-metastatic tissues. These findings were validated by quantitative real-time RT-PCR. Further studies on type XIV collagen are desired to verify its role as a prognostic factor and diagnosis marker for metastasis.
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spelling pubmed-45037642015-07-17 Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer Goto, Rieko Nakamura, Yasushi Takami, Tomonori Sanke, Tokio Tozuka, Zenzaburo PLoS One Research Article The purpose of this study was to develop quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the analysis of proteins involved in metastasis of breast cancer for diagnosis and determining disease prognosis, as well as to further our understand of metastatic mechanisms. We have previously demonstrated that the protein type XIV collagen may be specifically expressed in metastatic tissues by two dimensional LC-MS/MS. In this study, we developed quantitative LC-MS/MS methods for type XIV collagen. Type XIV collagen was quantified by analyzing 2 peptides generated by digesting type XIV collagen using stable isotope-labeled peptides. The individual concentrations were equivalent between 2 different peptides of type XIV collagen by evaluation of imprecise transitions and using the best transition for the peptide concentration. The results indicated that type XIV collagen is highly expressed in metastatic tissues of patients with massive lymph node involvement compared to non-metastatic tissues. These findings were validated by quantitative real-time RT-PCR. Further studies on type XIV collagen are desired to verify its role as a prognostic factor and diagnosis marker for metastasis. Public Library of Science 2015-07-15 /pmc/articles/PMC4503764/ /pubmed/26176947 http://dx.doi.org/10.1371/journal.pone.0130760 Text en © 2015 Goto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Goto, Rieko
Nakamura, Yasushi
Takami, Tomonori
Sanke, Tokio
Tozuka, Zenzaburo
Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title_full Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title_fullStr Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title_full_unstemmed Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title_short Quantitative LC-MS/MS Analysis of Proteins Involved in Metastasis of Breast Cancer
title_sort quantitative lc-ms/ms analysis of proteins involved in metastasis of breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503764/
https://www.ncbi.nlm.nih.gov/pubmed/26176947
http://dx.doi.org/10.1371/journal.pone.0130760
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