Development of a V(H)H-Based Erythropoietin Quantification Assay

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against...

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Detalles Bibliográficos
Autores principales: Kol, Stefan, Kallehauge, Thomas Beuchert, Adema, Simon, Hermans, Pim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503862/
https://www.ncbi.nlm.nih.gov/pubmed/25764454
http://dx.doi.org/10.1007/s12033-015-9860-7
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author Kol, Stefan
Kallehauge, Thomas Beuchert
Adema, Simon
Hermans, Pim
author_facet Kol, Stefan
Kallehauge, Thomas Beuchert
Adema, Simon
Hermans, Pim
author_sort Kol, Stefan
collection PubMed
description Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting.
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spelling pubmed-45038622015-07-17 Development of a V(H)H-Based Erythropoietin Quantification Assay Kol, Stefan Kallehauge, Thomas Beuchert Adema, Simon Hermans, Pim Mol Biotechnol Research Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting. Springer US 2015-03-13 2015 /pmc/articles/PMC4503862/ /pubmed/25764454 http://dx.doi.org/10.1007/s12033-015-9860-7 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research
Kol, Stefan
Kallehauge, Thomas Beuchert
Adema, Simon
Hermans, Pim
Development of a V(H)H-Based Erythropoietin Quantification Assay
title Development of a V(H)H-Based Erythropoietin Quantification Assay
title_full Development of a V(H)H-Based Erythropoietin Quantification Assay
title_fullStr Development of a V(H)H-Based Erythropoietin Quantification Assay
title_full_unstemmed Development of a V(H)H-Based Erythropoietin Quantification Assay
title_short Development of a V(H)H-Based Erythropoietin Quantification Assay
title_sort development of a v(h)h-based erythropoietin quantification assay
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503862/
https://www.ncbi.nlm.nih.gov/pubmed/25764454
http://dx.doi.org/10.1007/s12033-015-9860-7
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