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Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation
Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503951/ https://www.ncbi.nlm.nih.gov/pubmed/26179205 http://dx.doi.org/10.1038/srep12240 |
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author | Tang, Xianfeng Zhuang, Yamei Qi, Guang Wang, Dian Liu, Huanhuan Wang, Kairong Chai, Guohua Zhou, Gongke |
author_facet | Tang, Xianfeng Zhuang, Yamei Qi, Guang Wang, Dian Liu, Huanhuan Wang, Kairong Chai, Guohua Zhou, Gongke |
author_sort | Tang, Xianfeng |
collection | PubMed |
description | Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis. |
format | Online Article Text |
id | pubmed-4503951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45039512015-07-23 Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation Tang, Xianfeng Zhuang, Yamei Qi, Guang Wang, Dian Liu, Huanhuan Wang, Kairong Chai, Guohua Zhou, Gongke Sci Rep Article Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis. Nature Publishing Group 2015-07-16 /pmc/articles/PMC4503951/ /pubmed/26179205 http://dx.doi.org/10.1038/srep12240 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tang, Xianfeng Zhuang, Yamei Qi, Guang Wang, Dian Liu, Huanhuan Wang, Kairong Chai, Guohua Zhou, Gongke Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title | Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title_full | Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title_fullStr | Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title_full_unstemmed | Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title_short | Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
title_sort | poplar pdmyb221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503951/ https://www.ncbi.nlm.nih.gov/pubmed/26179205 http://dx.doi.org/10.1038/srep12240 |
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