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Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription

Ozone therapy is a modestly invasive procedure based on the regeneration capabilities of low ozone concentrations and used in medicine as an alternative/adjuvant treatment for different diseases. However, the cellular mechanisms accounting for the positive effects of mild ozonization are still large...

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Autores principales: Costanzo, M., Cisterna, B., Vella, A., Cestari, T., Covi, V., Tabaracci, G., Malatesta, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503975/
https://www.ncbi.nlm.nih.gov/pubmed/26150162
http://dx.doi.org/10.4081/ejh.2015.2515
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author Costanzo, M.
Cisterna, B.
Vella, A.
Cestari, T.
Covi, V.
Tabaracci, G.
Malatesta, M.
author_facet Costanzo, M.
Cisterna, B.
Vella, A.
Cestari, T.
Covi, V.
Tabaracci, G.
Malatesta, M.
author_sort Costanzo, M.
collection PubMed
description Ozone therapy is a modestly invasive procedure based on the regeneration capabilities of low ozone concentrations and used in medicine as an alternative/adjuvant treatment for different diseases. However, the cellular mechanisms accounting for the positive effects of mild ozonization are still largely unexplored. To this aim, in the present study the effects of low ozone concentrations (1 to 20 µg O(3)/mL O(2)) on structural and functional cell features have been investigated in vitro by using morphological, morphometrical, cytochemical and immunocytochemical techniques at bright field, fluorescence and transmission electron microscopy. Cells exposed to pure O(2) or air served as controls. The results demonstrated that the effects of ozone administration are dependent on gas concentration, and the cytoskeletal organization, mitochondrial activity and nuclear transcription may be differently affected. This suggests that, to ensure effective and permanent metabolic cell activation, ozone treatments should take into account the cytological and cytokinetic features of the different tissues.
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spelling pubmed-45039752015-08-11 Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription Costanzo, M. Cisterna, B. Vella, A. Cestari, T. Covi, V. Tabaracci, G. Malatesta, M. Eur J Histochem Original Paper Ozone therapy is a modestly invasive procedure based on the regeneration capabilities of low ozone concentrations and used in medicine as an alternative/adjuvant treatment for different diseases. However, the cellular mechanisms accounting for the positive effects of mild ozonization are still largely unexplored. To this aim, in the present study the effects of low ozone concentrations (1 to 20 µg O(3)/mL O(2)) on structural and functional cell features have been investigated in vitro by using morphological, morphometrical, cytochemical and immunocytochemical techniques at bright field, fluorescence and transmission electron microscopy. Cells exposed to pure O(2) or air served as controls. The results demonstrated that the effects of ozone administration are dependent on gas concentration, and the cytoskeletal organization, mitochondrial activity and nuclear transcription may be differently affected. This suggests that, to ensure effective and permanent metabolic cell activation, ozone treatments should take into account the cytological and cytokinetic features of the different tissues. PAGEPress Publications, Pavia, Italy 2015-04-21 /pmc/articles/PMC4503975/ /pubmed/26150162 http://dx.doi.org/10.4081/ejh.2015.2515 Text en ©Copyright M. Costanzo et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Costanzo, M.
Cisterna, B.
Vella, A.
Cestari, T.
Covi, V.
Tabaracci, G.
Malatesta, M.
Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title_full Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title_fullStr Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title_full_unstemmed Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title_short Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
title_sort low ozone concentrations stimulate cytoskeletal organization, mitochondrial activity and nuclear transcription
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503975/
https://www.ncbi.nlm.nih.gov/pubmed/26150162
http://dx.doi.org/10.4081/ejh.2015.2515
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