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Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for t...

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Autores principales: Camillo, Julceia, Leão, André P, Alves, Alexandre A, Formighieri, Eduardo F, Azevedo, Ana LS, Nunes, Juliana D, de Capdeville, Guy, de A Mattos, Jean K, Souza, Manoel T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504075/
https://www.ncbi.nlm.nih.gov/pubmed/26203259
http://dx.doi.org/10.4137/GEI.S15522
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author Camillo, Julceia
Leão, André P
Alves, Alexandre A
Formighieri, Eduardo F
Azevedo, Ana LS
Nunes, Juliana D
de Capdeville, Guy
de A Mattos, Jean K
Souza, Manoel T
author_facet Camillo, Julceia
Leão, André P
Alves, Alexandre A
Formighieri, Eduardo F
Azevedo, Ana LS
Nunes, Juliana D
de Capdeville, Guy
de A Mattos, Jean K
Souza, Manoel T
author_sort Camillo, Julceia
collection PubMed
description Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the parental species genomes. We estimated the genome size of three E. guineensis genotypes, five E. oleifera genotypes, and two interspecific hybrids genotypes. On average, the genome size of E. guineensis is 4.32 ± 0.173 pg, while that of E. oleifera is 4.43 ± 0.018 pg. This indicates that both genomes are similar in size, even though E. oleifera is in fact bigger. As expected, the hybrid genome size is around the average of the two genomes, 4.40 ± 0.016 pg. Additionally, we demonstrate that both species present around 38% of GC content. As our results contradict the currently available data on Elaeis spp. genome sizes, we propose that the actual genome size of the Elaeis species is around 4 pg and that American oil palm possesses a larger genome than African oil palm.
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spelling pubmed-45040752015-07-22 Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid Camillo, Julceia Leão, André P Alves, Alexandre A Formighieri, Eduardo F Azevedo, Ana LS Nunes, Juliana D de Capdeville, Guy de A Mattos, Jean K Souza, Manoel T Genomics Insights Original Research Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the parental species genomes. We estimated the genome size of three E. guineensis genotypes, five E. oleifera genotypes, and two interspecific hybrids genotypes. On average, the genome size of E. guineensis is 4.32 ± 0.173 pg, while that of E. oleifera is 4.43 ± 0.018 pg. This indicates that both genomes are similar in size, even though E. oleifera is in fact bigger. As expected, the hybrid genome size is around the average of the two genomes, 4.40 ± 0.016 pg. Additionally, we demonstrate that both species present around 38% of GC content. As our results contradict the currently available data on Elaeis spp. genome sizes, we propose that the actual genome size of the Elaeis species is around 4 pg and that American oil palm possesses a larger genome than African oil palm. Libertas Academica 2014-09-03 /pmc/articles/PMC4504075/ /pubmed/26203259 http://dx.doi.org/10.4137/GEI.S15522 Text en © 2014 the author(s), publisher and licensee Libertas Academica Ltd. This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 License.
spellingShingle Original Research
Camillo, Julceia
Leão, André P
Alves, Alexandre A
Formighieri, Eduardo F
Azevedo, Ana LS
Nunes, Juliana D
de Capdeville, Guy
de A Mattos, Jean K
Souza, Manoel T
Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title_full Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title_fullStr Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title_full_unstemmed Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title_short Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
title_sort reassessment of the genome size in elaeis guineensis and elaeis oleifera, and its interspecific hybrid
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504075/
https://www.ncbi.nlm.nih.gov/pubmed/26203259
http://dx.doi.org/10.4137/GEI.S15522
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