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Annexin A3 Regulates Early Blood Vessel Formation

Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (A...

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Detalles Bibliográficos
Autores principales: Meadows, Stryder M., Cleaver, Ondine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504506/
https://www.ncbi.nlm.nih.gov/pubmed/26182056
http://dx.doi.org/10.1371/journal.pone.0132580
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author Meadows, Stryder M.
Cleaver, Ondine
author_facet Meadows, Stryder M.
Cleaver, Ondine
author_sort Meadows, Stryder M.
collection PubMed
description Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology.
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spelling pubmed-45045062015-07-17 Annexin A3 Regulates Early Blood Vessel Formation Meadows, Stryder M. Cleaver, Ondine PLoS One Research Article Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology. Public Library of Science 2015-07-16 /pmc/articles/PMC4504506/ /pubmed/26182056 http://dx.doi.org/10.1371/journal.pone.0132580 Text en © 2015 Meadows, Cleaver http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Meadows, Stryder M.
Cleaver, Ondine
Annexin A3 Regulates Early Blood Vessel Formation
title Annexin A3 Regulates Early Blood Vessel Formation
title_full Annexin A3 Regulates Early Blood Vessel Formation
title_fullStr Annexin A3 Regulates Early Blood Vessel Formation
title_full_unstemmed Annexin A3 Regulates Early Blood Vessel Formation
title_short Annexin A3 Regulates Early Blood Vessel Formation
title_sort annexin a3 regulates early blood vessel formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504506/
https://www.ncbi.nlm.nih.gov/pubmed/26182056
http://dx.doi.org/10.1371/journal.pone.0132580
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