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Annexin A3 Regulates Early Blood Vessel Formation
Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (A...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504506/ https://www.ncbi.nlm.nih.gov/pubmed/26182056 http://dx.doi.org/10.1371/journal.pone.0132580 |
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author | Meadows, Stryder M. Cleaver, Ondine |
author_facet | Meadows, Stryder M. Cleaver, Ondine |
author_sort | Meadows, Stryder M. |
collection | PubMed |
description | Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology. |
format | Online Article Text |
id | pubmed-4504506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45045062015-07-17 Annexin A3 Regulates Early Blood Vessel Formation Meadows, Stryder M. Cleaver, Ondine PLoS One Research Article Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology. Public Library of Science 2015-07-16 /pmc/articles/PMC4504506/ /pubmed/26182056 http://dx.doi.org/10.1371/journal.pone.0132580 Text en © 2015 Meadows, Cleaver http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Meadows, Stryder M. Cleaver, Ondine Annexin A3 Regulates Early Blood Vessel Formation |
title | Annexin A3 Regulates Early Blood Vessel Formation |
title_full | Annexin A3 Regulates Early Blood Vessel Formation |
title_fullStr | Annexin A3 Regulates Early Blood Vessel Formation |
title_full_unstemmed | Annexin A3 Regulates Early Blood Vessel Formation |
title_short | Annexin A3 Regulates Early Blood Vessel Formation |
title_sort | annexin a3 regulates early blood vessel formation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504506/ https://www.ncbi.nlm.nih.gov/pubmed/26182056 http://dx.doi.org/10.1371/journal.pone.0132580 |
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