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Back to Basics – The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities

DNA extraction and primer choice have a large effect on the observed community structure in all microbial amplicon sequencing analyses. Although the biases are well known, no comprehensive analysis has been conducted in activated sludge communities. In this study we systematically explored the impac...

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Detalles Bibliográficos
Autores principales:	Albertsen, Mads, Karst, Søren M., Ziegler, Anja S., Kirkegaard, Rasmus H., Nielsen, Per H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4504704/ https://www.ncbi.nlm.nih.gov/pubmed/26182345 http://dx.doi.org/10.1371/journal.pone.0132783

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