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Optimization of Protein Extraction for Lichen Thalli

Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these proble...

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Detalles Bibliográficos
Autores principales: Kondratiuk, Anna S., Savchuk, Oleksiy M., Hur, Jae-Seoun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505004/
https://www.ncbi.nlm.nih.gov/pubmed/26190923
http://dx.doi.org/10.5941/MYCO.2015.43.2.157
Descripción
Sumario:Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.