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Optimization of Protein Extraction for Lichen Thalli
Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these proble...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Mycology
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505004/ https://www.ncbi.nlm.nih.gov/pubmed/26190923 http://dx.doi.org/10.5941/MYCO.2015.43.2.157 |
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author | Kondratiuk, Anna S. Savchuk, Oleksiy M. Hur, Jae-Seoun |
author_facet | Kondratiuk, Anna S. Savchuk, Oleksiy M. Hur, Jae-Seoun |
author_sort | Kondratiuk, Anna S. |
collection | PubMed |
description | Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study. |
format | Online Article Text |
id | pubmed-4505004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Society of Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-45050042015-07-17 Optimization of Protein Extraction for Lichen Thalli Kondratiuk, Anna S. Savchuk, Oleksiy M. Hur, Jae-Seoun Mycobiology Research Article Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study. The Korean Society of Mycology 2015-06 2015-06-30 /pmc/articles/PMC4505004/ /pubmed/26190923 http://dx.doi.org/10.5941/MYCO.2015.43.2.157 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kondratiuk, Anna S. Savchuk, Oleksiy M. Hur, Jae-Seoun Optimization of Protein Extraction for Lichen Thalli |
title | Optimization of Protein Extraction for Lichen Thalli |
title_full | Optimization of Protein Extraction for Lichen Thalli |
title_fullStr | Optimization of Protein Extraction for Lichen Thalli |
title_full_unstemmed | Optimization of Protein Extraction for Lichen Thalli |
title_short | Optimization of Protein Extraction for Lichen Thalli |
title_sort | optimization of protein extraction for lichen thalli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505004/ https://www.ncbi.nlm.nih.gov/pubmed/26190923 http://dx.doi.org/10.5941/MYCO.2015.43.2.157 |
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