A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector

Fluorescent tracking gene delivery could provide us with a better understanding of the critical steps in the transfection process. However, for in vivo tracking applications, a small diameter (<10 nm) is one of the rigorous requirements for tracking vectors. Herein, we have demonstrated a new par...

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Autores principales: Qiu, Kangqiang, Yu, Bole, Huang, Huaiyi, Zhang, Pingyu, Huang, Juanjuan, Zou, Shanshan, Chen, Yu, Ji, Liangnian, Chao, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505312/
https://www.ncbi.nlm.nih.gov/pubmed/26185052
http://dx.doi.org/10.1038/srep10707
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author Qiu, Kangqiang
Yu, Bole
Huang, Huaiyi
Zhang, Pingyu
Huang, Juanjuan
Zou, Shanshan
Chen, Yu
Ji, Liangnian
Chao, Hui
author_facet Qiu, Kangqiang
Yu, Bole
Huang, Huaiyi
Zhang, Pingyu
Huang, Juanjuan
Zou, Shanshan
Chen, Yu
Ji, Liangnian
Chao, Hui
author_sort Qiu, Kangqiang
collection PubMed
description Fluorescent tracking gene delivery could provide us with a better understanding of the critical steps in the transfection process. However, for in vivo tracking applications, a small diameter (<10 nm) is one of the rigorous requirements for tracking vectors. Herein, we have demonstrated a new paradigm for two-photon tracking gene delivery based on a dendritic nano-sized hexanuclear ruthenium(II) polypyridyl complex. Because this metallodendrimer has a multivalent periphery, the complex, which is 6.1 nm, showed high stability and excellent dispersibility and could stepwise condense DNA in vitro. With the outstanding photochemical properties of Ru(II) polypyridyl, this complex could track gene delivery in vivo using one- and two-photon imaging.
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spelling pubmed-45053122015-07-23 A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector Qiu, Kangqiang Yu, Bole Huang, Huaiyi Zhang, Pingyu Huang, Juanjuan Zou, Shanshan Chen, Yu Ji, Liangnian Chao, Hui Sci Rep Article Fluorescent tracking gene delivery could provide us with a better understanding of the critical steps in the transfection process. However, for in vivo tracking applications, a small diameter (<10 nm) is one of the rigorous requirements for tracking vectors. Herein, we have demonstrated a new paradigm for two-photon tracking gene delivery based on a dendritic nano-sized hexanuclear ruthenium(II) polypyridyl complex. Because this metallodendrimer has a multivalent periphery, the complex, which is 6.1 nm, showed high stability and excellent dispersibility and could stepwise condense DNA in vitro. With the outstanding photochemical properties of Ru(II) polypyridyl, this complex could track gene delivery in vivo using one- and two-photon imaging. Nature Publishing Group 2015-07-17 /pmc/articles/PMC4505312/ /pubmed/26185052 http://dx.doi.org/10.1038/srep10707 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Qiu, Kangqiang
Yu, Bole
Huang, Huaiyi
Zhang, Pingyu
Huang, Juanjuan
Zou, Shanshan
Chen, Yu
Ji, Liangnian
Chao, Hui
A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title_full A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title_fullStr A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title_full_unstemmed A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title_short A dendritic nano-sized hexanuclear ruthenium(II) complex as a one- and two-photon luminescent tracking non-viral gene vector
title_sort dendritic nano-sized hexanuclear ruthenium(ii) complex as a one- and two-photon luminescent tracking non-viral gene vector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505312/
https://www.ncbi.nlm.nih.gov/pubmed/26185052
http://dx.doi.org/10.1038/srep10707
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