Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath

A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results i...

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Autores principales: Kasoji, Sandeep K., Pattenden, Samantha G., Malc, Ewa P., Jayakody, Chatura N., Tsuruta, James K., Mieczkowski, Piotr A., Janzen, William P., Dayton, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505845/
https://www.ncbi.nlm.nih.gov/pubmed/26186461
http://dx.doi.org/10.1371/journal.pone.0133014
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author Kasoji, Sandeep K.
Pattenden, Samantha G.
Malc, Ewa P.
Jayakody, Chatura N.
Tsuruta, James K.
Mieczkowski, Piotr A.
Janzen, William P.
Dayton, Paul A.
author_facet Kasoji, Sandeep K.
Pattenden, Samantha G.
Malc, Ewa P.
Jayakody, Chatura N.
Tsuruta, James K.
Mieczkowski, Piotr A.
Janzen, William P.
Dayton, Paul A.
author_sort Kasoji, Sandeep K.
collection PubMed
description A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results indistinguishable from DNA samples fragmented in dedicated commercial acoustic sonication equipment, and with higher throughput. This technique thus enables widespread access to fast bench-top genomic DNA fragmentation.
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spelling pubmed-45058452015-07-23 Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath Kasoji, Sandeep K. Pattenden, Samantha G. Malc, Ewa P. Jayakody, Chatura N. Tsuruta, James K. Mieczkowski, Piotr A. Janzen, William P. Dayton, Paul A. PLoS One Research Article A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results indistinguishable from DNA samples fragmented in dedicated commercial acoustic sonication equipment, and with higher throughput. This technique thus enables widespread access to fast bench-top genomic DNA fragmentation. Public Library of Science 2015-07-17 /pmc/articles/PMC4505845/ /pubmed/26186461 http://dx.doi.org/10.1371/journal.pone.0133014 Text en © 2015 Kasoji et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kasoji, Sandeep K.
Pattenden, Samantha G.
Malc, Ewa P.
Jayakody, Chatura N.
Tsuruta, James K.
Mieczkowski, Piotr A.
Janzen, William P.
Dayton, Paul A.
Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title_full Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title_fullStr Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title_full_unstemmed Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title_short Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
title_sort cavitation enhancing nanodroplets mediate efficient dna fragmentation in a bench top ultrasonic water bath
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505845/
https://www.ncbi.nlm.nih.gov/pubmed/26186461
http://dx.doi.org/10.1371/journal.pone.0133014
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