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Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath

A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results i...

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Detalles Bibliográficos
Autores principales: Kasoji, Sandeep K., Pattenden, Samantha G., Malc, Ewa P., Jayakody, Chatura N., Tsuruta, James K., Mieczkowski, Piotr A., Janzen, William P., Dayton, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505845/
https://www.ncbi.nlm.nih.gov/pubmed/26186461
http://dx.doi.org/10.1371/journal.pone.0133014