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Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results i...
Autores principales: | Kasoji, Sandeep K., Pattenden, Samantha G., Malc, Ewa P., Jayakody, Chatura N., Tsuruta, James K., Mieczkowski, Piotr A., Janzen, William P., Dayton, Paul A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505845/ https://www.ncbi.nlm.nih.gov/pubmed/26186461 http://dx.doi.org/10.1371/journal.pone.0133014 |
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