Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)

RNA-sequencing of a case of acute myeloid leukemia with the bone marrow karyotype 46,XY,t(2;14)(q22;q32)[5]/47,XY,idem,+?4,del(6)(q13q21)[cp6]/46,XY[4] showed that the t(2;14) generated a ZEB2-BCL11B chimera in which exon 2 of ZEB2 (nucleotide 595 in the sequence with accession number NM_014795.3) w...

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Autores principales: Torkildsen, Synne, Gorunova, Ludmila, Beiske, Klaus, Tjønnfjord, Geir E., Heim, Sverre, Panagopoulos, Ioannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505893/
https://www.ncbi.nlm.nih.gov/pubmed/26186352
http://dx.doi.org/10.1371/journal.pone.0132736
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author Torkildsen, Synne
Gorunova, Ludmila
Beiske, Klaus
Tjønnfjord, Geir E.
Heim, Sverre
Panagopoulos, Ioannis
author_facet Torkildsen, Synne
Gorunova, Ludmila
Beiske, Klaus
Tjønnfjord, Geir E.
Heim, Sverre
Panagopoulos, Ioannis
author_sort Torkildsen, Synne
collection PubMed
description RNA-sequencing of a case of acute myeloid leukemia with the bone marrow karyotype 46,XY,t(2;14)(q22;q32)[5]/47,XY,idem,+?4,del(6)(q13q21)[cp6]/46,XY[4] showed that the t(2;14) generated a ZEB2-BCL11B chimera in which exon 2 of ZEB2 (nucleotide 595 in the sequence with accession number NM_014795.3) was fused to exon 2 of BCL11B (nucleotide 554 in the sequence with accession number NM_022898.2). RT-PCR together with Sanger sequencing verified the presence of the above-mentioned fusion transcript. All functional domains of BCL11B are retained in the chimeric protein. Abnormal expression of BCL11B coding regions subjected to control by the ZEB2 promoter seems to be the leukemogenic mechanism behind the translocation.
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spelling pubmed-45058932015-07-23 Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32) Torkildsen, Synne Gorunova, Ludmila Beiske, Klaus Tjønnfjord, Geir E. Heim, Sverre Panagopoulos, Ioannis PLoS One Research Article RNA-sequencing of a case of acute myeloid leukemia with the bone marrow karyotype 46,XY,t(2;14)(q22;q32)[5]/47,XY,idem,+?4,del(6)(q13q21)[cp6]/46,XY[4] showed that the t(2;14) generated a ZEB2-BCL11B chimera in which exon 2 of ZEB2 (nucleotide 595 in the sequence with accession number NM_014795.3) was fused to exon 2 of BCL11B (nucleotide 554 in the sequence with accession number NM_022898.2). RT-PCR together with Sanger sequencing verified the presence of the above-mentioned fusion transcript. All functional domains of BCL11B are retained in the chimeric protein. Abnormal expression of BCL11B coding regions subjected to control by the ZEB2 promoter seems to be the leukemogenic mechanism behind the translocation. Public Library of Science 2015-07-17 /pmc/articles/PMC4505893/ /pubmed/26186352 http://dx.doi.org/10.1371/journal.pone.0132736 Text en © 2015 Torkildsen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Torkildsen, Synne
Gorunova, Ludmila
Beiske, Klaus
Tjønnfjord, Geir E.
Heim, Sverre
Panagopoulos, Ioannis
Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title_full Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title_fullStr Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title_full_unstemmed Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title_short Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14)(q22;q32)
title_sort novel zeb2-bcl11b fusion gene identified by rna-sequencing in acute myeloid leukemia with t(2;14)(q22;q32)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4505893/
https://www.ncbi.nlm.nih.gov/pubmed/26186352
http://dx.doi.org/10.1371/journal.pone.0132736
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