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Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia

BACKGROUND: Perinatal asphyxia is an important cause of mortality and permanent neurological and developmental deficit. Early and accurate diagnosis would help to establish the likely prognosis and may also help in determining the most appropriate treatment. Studies in experimental animal models sug...

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Autores principales: Boskabadi, Hassan, Omidian, Masoud, Tavallai, Shima, Mohammadi, Shabnam, Parizadeh, Mostafa, Ghayour Mobarhan, Majid, Ferns, Gordon AA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506008/
https://www.ncbi.nlm.nih.gov/pubmed/26196004
http://dx.doi.org/10.5812/ijp.381
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author Boskabadi, Hassan
Omidian, Masoud
Tavallai, Shima
Mohammadi, Shabnam
Parizadeh, Mostafa
Ghayour Mobarhan, Majid
Ferns, Gordon AA
author_facet Boskabadi, Hassan
Omidian, Masoud
Tavallai, Shima
Mohammadi, Shabnam
Parizadeh, Mostafa
Ghayour Mobarhan, Majid
Ferns, Gordon AA
author_sort Boskabadi, Hassan
collection PubMed
description BACKGROUND: Perinatal asphyxia is an important cause of mortality and permanent neurological and developmental deficit. Early and accurate diagnosis would help to establish the likely prognosis and may also help in determining the most appropriate treatment. Studies in experimental animal models suggest that a protein called Hsp70 may be a good and potentially useful marker of cellular stress that may be clinically useful in determining the presence of neonatal asphyxia. OBJECTIVES: Regarding the importance of early and accurate diagnosis of asphyxia, we conducted this study, which is the first investigation of the comparison of the serum Hsp70 antigen level between asphyxiated and healthy infants. PATIENTS AND METHODS: In this observational study, the serum concentrations of Hsp70 antigen were compared between neonates suffering from perinatal asphyxia (n = 50) and normal neonates (n = 51). The inclusion criteria for the cases were neonates who had reached term and had at least two clinical criteria of asphyxia. Exclusion criteria were babies with gestational age < 37 weeks, infants with congenital abnormalities or positive blood culture. Exclusion criteria in this group were the requirement to hospital stay during first week of the life or babies whose mothers had difficulties during pregnancy or delivery. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group consisted of healthy term neonates without problems and normal delivery process in the first week of life. The cord blood was taken during labor to measure Hsp70 antigen level by using an in-house ELISA (The enzyme-linked immunosorbent assay). RESULTS: The median values of serum anti Hsp70 titers were significantly higher in asphyxiated neonates compared with non-asphyxiated neonates (0.36 [0.04 - 1.14] vs 0.24 [0.01 - 0.63]). At cutoff point = 0.3125 ng/mL, sensitivity was 58% and specificity 76% based on ROC curve. CONCLUSIONS: A significant difference between the serum concentrations of Hsp70 of the control and patient group was observed in this study. It is inferred serum concentrations of Hsp70 antigen may be a useful marker for the early diagnosis of that prenatal hypoxia.
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spelling pubmed-45060082015-07-20 Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia Boskabadi, Hassan Omidian, Masoud Tavallai, Shima Mohammadi, Shabnam Parizadeh, Mostafa Ghayour Mobarhan, Majid Ferns, Gordon AA Iran J Pediatr Research Article BACKGROUND: Perinatal asphyxia is an important cause of mortality and permanent neurological and developmental deficit. Early and accurate diagnosis would help to establish the likely prognosis and may also help in determining the most appropriate treatment. Studies in experimental animal models suggest that a protein called Hsp70 may be a good and potentially useful marker of cellular stress that may be clinically useful in determining the presence of neonatal asphyxia. OBJECTIVES: Regarding the importance of early and accurate diagnosis of asphyxia, we conducted this study, which is the first investigation of the comparison of the serum Hsp70 antigen level between asphyxiated and healthy infants. PATIENTS AND METHODS: In this observational study, the serum concentrations of Hsp70 antigen were compared between neonates suffering from perinatal asphyxia (n = 50) and normal neonates (n = 51). The inclusion criteria for the cases were neonates who had reached term and had at least two clinical criteria of asphyxia. Exclusion criteria were babies with gestational age < 37 weeks, infants with congenital abnormalities or positive blood culture. Exclusion criteria in this group were the requirement to hospital stay during first week of the life or babies whose mothers had difficulties during pregnancy or delivery. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group consisted of healthy term neonates without problems and normal delivery process in the first week of life. The cord blood was taken during labor to measure Hsp70 antigen level by using an in-house ELISA (The enzyme-linked immunosorbent assay). RESULTS: The median values of serum anti Hsp70 titers were significantly higher in asphyxiated neonates compared with non-asphyxiated neonates (0.36 [0.04 - 1.14] vs 0.24 [0.01 - 0.63]). At cutoff point = 0.3125 ng/mL, sensitivity was 58% and specificity 76% based on ROC curve. CONCLUSIONS: A significant difference between the serum concentrations of Hsp70 of the control and patient group was observed in this study. It is inferred serum concentrations of Hsp70 antigen may be a useful marker for the early diagnosis of that prenatal hypoxia. Kowsar 2015-04-18 2015-04 /pmc/articles/PMC4506008/ /pubmed/26196004 http://dx.doi.org/10.5812/ijp.381 Text en Copyright © 2015, Growth & Development Research Center. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Boskabadi, Hassan
Omidian, Masoud
Tavallai, Shima
Mohammadi, Shabnam
Parizadeh, Mostafa
Ghayour Mobarhan, Majid
Ferns, Gordon AA
Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title_full Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title_fullStr Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title_full_unstemmed Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title_short Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia
title_sort serum hsp70 antigen: early diagnosis marker in perinatal asphyxia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506008/
https://www.ncbi.nlm.nih.gov/pubmed/26196004
http://dx.doi.org/10.5812/ijp.381
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