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Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury
BACKGROUND/AIM: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cel...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506035/ https://www.ncbi.nlm.nih.gov/pubmed/26203260 http://dx.doi.org/10.2147/OTT.S86743 |
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author | Mizuno, Tomohiro Sato, Waichi Ishikawa, Kazuhiro Terao, Yuki Takahashi, Kazuo Noda, Yukihiro Yuzawa, Yukio Nagamatsu, Tadashi |
author_facet | Mizuno, Tomohiro Sato, Waichi Ishikawa, Kazuhiro Terao, Yuki Takahashi, Kazuo Noda, Yukihiro Yuzawa, Yukio Nagamatsu, Tadashi |
author_sort | Mizuno, Tomohiro |
collection | PubMed |
description | BACKGROUND/AIM: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. MATERIALS AND METHODS: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. RESULTS: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. CONCLUSION: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury. |
format | Online Article Text |
id | pubmed-4506035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45060352015-07-22 Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury Mizuno, Tomohiro Sato, Waichi Ishikawa, Kazuhiro Terao, Yuki Takahashi, Kazuo Noda, Yukihiro Yuzawa, Yukio Nagamatsu, Tadashi Onco Targets Ther Original Research BACKGROUND/AIM: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. MATERIALS AND METHODS: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. RESULTS: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. CONCLUSION: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury. Dove Medical Press 2015-07-10 /pmc/articles/PMC4506035/ /pubmed/26203260 http://dx.doi.org/10.2147/OTT.S86743 Text en © 2015 Mizuno et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Mizuno, Tomohiro Sato, Waichi Ishikawa, Kazuhiro Terao, Yuki Takahashi, Kazuo Noda, Yukihiro Yuzawa, Yukio Nagamatsu, Tadashi Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title | Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title_full | Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title_fullStr | Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title_full_unstemmed | Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title_short | Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury |
title_sort | significance of downregulation of renal organic cation transporter (slc47a1) in cisplatin-induced proximal tubular injury |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506035/ https://www.ncbi.nlm.nih.gov/pubmed/26203260 http://dx.doi.org/10.2147/OTT.S86743 |
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