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Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
In this research, the wheat cultivar 'Lovrin 10' and Puccinia triticina races 165 and 260 were used to constitute compatible and incompatible combinations to investigate the relationship between NO and H(2)O(2) and between NO and calcium (Ca(2+)) signaling in the cell defense process by ph...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506137/ https://www.ncbi.nlm.nih.gov/pubmed/26185989 http://dx.doi.org/10.1371/journal.pone.0132265 |
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author | Qiao, Mei Sun, Jiawei Liu, Na Sun, Tianjie Liu, Gang Han, Shengfang Hou, Chunyan Wang, Dongmei |
author_facet | Qiao, Mei Sun, Jiawei Liu, Na Sun, Tianjie Liu, Gang Han, Shengfang Hou, Chunyan Wang, Dongmei |
author_sort | Qiao, Mei |
collection | PubMed |
description | In this research, the wheat cultivar 'Lovrin 10' and Puccinia triticina races 165 and 260 were used to constitute compatible and incompatible combinations to investigate the relationship between NO and H(2)O(2) and between NO and calcium (Ca(2+)) signaling in the cell defense process by pharmacological means. The specific fluorescent probe DAF-FM DA was coupled with confocal laser scanning microscopy and used to label intracellular nitric oxide (NO) and monitoring the real-time NO dynamics during the processes of wheat defense response triggered by P. triticina infection. The results showed that at 4 h after inoculation, weak green fluorescence was observed in the stomatal guard cells at the P. triticina infection site in the incompatible combination, which indicates a small amount of NO production. Twelve hours after inoculation, the fluorescence of NO in- cell adjacent to the stomata gradually intensified, and the NO fluorescent area also expanded continuously; the green fluorescence primarily occurred in the cells undergoing a hypersensitive response (HR) at 24–72 h after inoculation. For the compatible combination, however, a small amount of green fluorescence was observed in stomata where the pathogenic contact occurred at 4 h after inoculation, and fluorescence was not observed thereafter. Injections of the NO scavenger c-PTIO prior to inoculation postponed the onset of NO production to 48 h after inoculation and suppressed HR advancement. The injection of imidazole, a NADPH oxidase inhibitor, or EGTA, an extracellular calcium chelator, in the leaves prior to inoculation, delayed the onset of NO production in the incompatible combination and suppressed HR advancement. Combined with our previous results, it could be concluded that, Ca(2+) and hydrogen peroxide (H(2)O(2)) are involved in upstream of NO production to induce the HR cell death during P. triticina infection, and Ca(2+), NO and H(2)O(2) are jointly involved in the signal transduction process of HR in the interaction system. |
format | Online Article Text |
id | pubmed-4506137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45061372015-07-23 Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina Qiao, Mei Sun, Jiawei Liu, Na Sun, Tianjie Liu, Gang Han, Shengfang Hou, Chunyan Wang, Dongmei PLoS One Research Article In this research, the wheat cultivar 'Lovrin 10' and Puccinia triticina races 165 and 260 were used to constitute compatible and incompatible combinations to investigate the relationship between NO and H(2)O(2) and between NO and calcium (Ca(2+)) signaling in the cell defense process by pharmacological means. The specific fluorescent probe DAF-FM DA was coupled with confocal laser scanning microscopy and used to label intracellular nitric oxide (NO) and monitoring the real-time NO dynamics during the processes of wheat defense response triggered by P. triticina infection. The results showed that at 4 h after inoculation, weak green fluorescence was observed in the stomatal guard cells at the P. triticina infection site in the incompatible combination, which indicates a small amount of NO production. Twelve hours after inoculation, the fluorescence of NO in- cell adjacent to the stomata gradually intensified, and the NO fluorescent area also expanded continuously; the green fluorescence primarily occurred in the cells undergoing a hypersensitive response (HR) at 24–72 h after inoculation. For the compatible combination, however, a small amount of green fluorescence was observed in stomata where the pathogenic contact occurred at 4 h after inoculation, and fluorescence was not observed thereafter. Injections of the NO scavenger c-PTIO prior to inoculation postponed the onset of NO production to 48 h after inoculation and suppressed HR advancement. The injection of imidazole, a NADPH oxidase inhibitor, or EGTA, an extracellular calcium chelator, in the leaves prior to inoculation, delayed the onset of NO production in the incompatible combination and suppressed HR advancement. Combined with our previous results, it could be concluded that, Ca(2+) and hydrogen peroxide (H(2)O(2)) are involved in upstream of NO production to induce the HR cell death during P. triticina infection, and Ca(2+), NO and H(2)O(2) are jointly involved in the signal transduction process of HR in the interaction system. Public Library of Science 2015-07-17 /pmc/articles/PMC4506137/ /pubmed/26185989 http://dx.doi.org/10.1371/journal.pone.0132265 Text en © 2015 Qiao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Qiao, Mei Sun, Jiawei Liu, Na Sun, Tianjie Liu, Gang Han, Shengfang Hou, Chunyan Wang, Dongmei Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina |
title | Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
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title_full | Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
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title_fullStr | Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
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title_full_unstemmed | Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
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title_short | Changes of Nitric Oxide and Its Relationship with H(2)O(2) and Ca(2+) in Defense Interactions between Wheat and Puccinia Triticina
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title_sort | changes of nitric oxide and its relationship with h(2)o(2) and ca(2+) in defense interactions between wheat and puccinia triticina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506137/ https://www.ncbi.nlm.nih.gov/pubmed/26185989 http://dx.doi.org/10.1371/journal.pone.0132265 |
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