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20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation

BACKGROUND: Antiallergic effect of 20(S)-protopanaxatriol (PPT), an intestinal metabolite of ginseng saponins, was investigated in guinea pig lung mast cells and mouse bone marrow-derived mast cells activated by a specific antigen/antibody reaction. METHODS: Increasing concentrations of PPT were pre...

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Autores principales: Kim, Dae Yong, Ro, Jai Youl, Lee, Chang Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506377/
https://www.ncbi.nlm.nih.gov/pubmed/26199549
http://dx.doi.org/10.1016/j.jgr.2014.11.001
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author Kim, Dae Yong
Ro, Jai Youl
Lee, Chang Ho
author_facet Kim, Dae Yong
Ro, Jai Youl
Lee, Chang Ho
author_sort Kim, Dae Yong
collection PubMed
description BACKGROUND: Antiallergic effect of 20(S)-protopanaxatriol (PPT), an intestinal metabolite of ginseng saponins, was investigated in guinea pig lung mast cells and mouse bone marrow-derived mast cells activated by a specific antigen/antibody reaction. METHODS: Increasing concentrations of PPT were pretreated 5 min prior to antigen stimulation, and various inflammatory mediator releases and their relevant cellular signaling events were measured in those cells. RESULTS: PPT dose-dependently reduced the release of histamine and leukotrienes in both types of mast cells. Especially, in activated bone marrow-derived mast cells, PPT inhibited the expression of Syk protein, cytokine mRNA, cyclooxygenase-1/2, and phospholipase A(2) (PLA(2)), as well as the activities of various protein kinase C isoforms, mitogen-activated protein kinases, PLA(2), and transcription factors (nuclear factor-κB and activator protein-1). CONCLUSION: PPT reduces the release of inflammatory mediators via inhibiting multiple cellular signaling pathways comprising the Ca(2+) influx, protein kinase C, and PLA(2), which are propagated by Syk activation upon allergic stimulation of mast cells.
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spelling pubmed-45063772015-07-21 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation Kim, Dae Yong Ro, Jai Youl Lee, Chang Ho J Ginseng Res Research Article BACKGROUND: Antiallergic effect of 20(S)-protopanaxatriol (PPT), an intestinal metabolite of ginseng saponins, was investigated in guinea pig lung mast cells and mouse bone marrow-derived mast cells activated by a specific antigen/antibody reaction. METHODS: Increasing concentrations of PPT were pretreated 5 min prior to antigen stimulation, and various inflammatory mediator releases and their relevant cellular signaling events were measured in those cells. RESULTS: PPT dose-dependently reduced the release of histamine and leukotrienes in both types of mast cells. Especially, in activated bone marrow-derived mast cells, PPT inhibited the expression of Syk protein, cytokine mRNA, cyclooxygenase-1/2, and phospholipase A(2) (PLA(2)), as well as the activities of various protein kinase C isoforms, mitogen-activated protein kinases, PLA(2), and transcription factors (nuclear factor-κB and activator protein-1). CONCLUSION: PPT reduces the release of inflammatory mediators via inhibiting multiple cellular signaling pathways comprising the Ca(2+) influx, protein kinase C, and PLA(2), which are propagated by Syk activation upon allergic stimulation of mast cells. Elsevier 2015-07 2014-11-24 /pmc/articles/PMC4506377/ /pubmed/26199549 http://dx.doi.org/10.1016/j.jgr.2014.11.001 Text en Copyright © 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Research Article
Kim, Dae Yong
Ro, Jai Youl
Lee, Chang Ho
20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title_full 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title_fullStr 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title_full_unstemmed 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title_short 20(S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin E-mediated mast cell activation
title_sort 20(s)-protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin e-mediated mast cell activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506377/
https://www.ncbi.nlm.nih.gov/pubmed/26199549
http://dx.doi.org/10.1016/j.jgr.2014.11.001
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