Cargando…
Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens
BACKGROUND: Hsf4 is closely related to the development of cataract. However, the molecular mechanisms remain unknown. This study aimed to explore the molecular mechanisms that how Hsf4 mutations influence development of lens and thus lead to cataract in mouse. METHODS: The mRNA expression profile of...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506420/ https://www.ncbi.nlm.nih.gov/pubmed/26187041 http://dx.doi.org/10.1186/s12886-015-0066-3 |
_version_ | 1782381681195352064 |
---|---|
author | Zhao, Wenjuan Zhao, Wenqing Zhao, Jun Wang, Dong Li, Jinghai |
author_facet | Zhao, Wenjuan Zhao, Wenqing Zhao, Jun Wang, Dong Li, Jinghai |
author_sort | Zhao, Wenjuan |
collection | PubMed |
description | BACKGROUND: Hsf4 is closely related to the development of cataract. However, the molecular mechanisms remain unknown. This study aimed to explore the molecular mechanisms that how Hsf4 mutations influence development of lens and thus lead to cataract in mouse. METHODS: The mRNA expression profile of mouse tissue samples from Hsf4-null and wile-type lenses was downloaded from Gene Expression Omnibus database. Then the LIMMA package was used to screen differentially expressed genes (DEGs) and DAVID was applied to identify the significantly enriched Gene Ontology (GO) categories for DEGs. Furthermore, the protein-protein interaction (PPI) network of DEGs was constructed using Cytoscape and the key modules were selected from the PPI network based on the MCODE analysis. RESULTS: A total of 216 DEGs were screened, including 51 up- and 165 down-regulated genes. Meanwhile, nine GO terms were obtained, and DEGs such as SGK1, CRY2 and REV1 were enriched in response to DNA damage stimulus. Furthermore, 89 DEGs and 99 gene pairs were mapped into the PPI network and Ubc was the hob node. Two key modules, which contained the genes (e.g. Ubc, Egr1, Ptgs2, Hmox1, Cd44, Btg2, Cyr61 and Fos) were related to response to DNA damage stimulus. CONCLUSIONS: The deletion of Hsf4 affects the expression of many genes, such as Ubc, Ptgs2, Egr1 and Fos. These genes may be involved in the development of cataract and could be used as therapeutic targets for cataract. |
format | Online Article Text |
id | pubmed-4506420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45064202015-07-19 Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens Zhao, Wenjuan Zhao, Wenqing Zhao, Jun Wang, Dong Li, Jinghai BMC Ophthalmol Research Article BACKGROUND: Hsf4 is closely related to the development of cataract. However, the molecular mechanisms remain unknown. This study aimed to explore the molecular mechanisms that how Hsf4 mutations influence development of lens and thus lead to cataract in mouse. METHODS: The mRNA expression profile of mouse tissue samples from Hsf4-null and wile-type lenses was downloaded from Gene Expression Omnibus database. Then the LIMMA package was used to screen differentially expressed genes (DEGs) and DAVID was applied to identify the significantly enriched Gene Ontology (GO) categories for DEGs. Furthermore, the protein-protein interaction (PPI) network of DEGs was constructed using Cytoscape and the key modules were selected from the PPI network based on the MCODE analysis. RESULTS: A total of 216 DEGs were screened, including 51 up- and 165 down-regulated genes. Meanwhile, nine GO terms were obtained, and DEGs such as SGK1, CRY2 and REV1 were enriched in response to DNA damage stimulus. Furthermore, 89 DEGs and 99 gene pairs were mapped into the PPI network and Ubc was the hob node. Two key modules, which contained the genes (e.g. Ubc, Egr1, Ptgs2, Hmox1, Cd44, Btg2, Cyr61 and Fos) were related to response to DNA damage stimulus. CONCLUSIONS: The deletion of Hsf4 affects the expression of many genes, such as Ubc, Ptgs2, Egr1 and Fos. These genes may be involved in the development of cataract and could be used as therapeutic targets for cataract. BioMed Central 2015-07-18 /pmc/articles/PMC4506420/ /pubmed/26187041 http://dx.doi.org/10.1186/s12886-015-0066-3 Text en © Zhao et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zhao, Wenjuan Zhao, Wenqing Zhao, Jun Wang, Dong Li, Jinghai Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title | Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title_full | Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title_fullStr | Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title_full_unstemmed | Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title_short | Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens |
title_sort | screening of potential target genes for cataract by analyzing mrna expression profile of mouse hsf4-null lens |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506420/ https://www.ncbi.nlm.nih.gov/pubmed/26187041 http://dx.doi.org/10.1186/s12886-015-0066-3 |
work_keys_str_mv | AT zhaowenjuan screeningofpotentialtargetgenesforcataractbyanalyzingmrnaexpressionprofileofmousehsf4nulllens AT zhaowenqing screeningofpotentialtargetgenesforcataractbyanalyzingmrnaexpressionprofileofmousehsf4nulllens AT zhaojun screeningofpotentialtargetgenesforcataractbyanalyzingmrnaexpressionprofileofmousehsf4nulllens AT wangdong screeningofpotentialtargetgenesforcataractbyanalyzingmrnaexpressionprofileofmousehsf4nulllens AT lijinghai screeningofpotentialtargetgenesforcataractbyanalyzingmrnaexpressionprofileofmousehsf4nulllens |