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Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR
MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perfor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506552/ https://www.ncbi.nlm.nih.gov/pubmed/26138755 http://dx.doi.org/10.1038/ncomms8693 |
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author | Suh, Yoon Seok Bhat, Shreelatha Hong, Seung-Hyun Shin, Minjung Bahk, Suhyoung Cho, Kyung Sang Kim, Seung-Whan Lee, Kyu-Sun Kim, Young-Joon Jones, Walton D. Yu, Kweon |
author_facet | Suh, Yoon Seok Bhat, Shreelatha Hong, Seung-Hyun Shin, Minjung Bahk, Suhyoung Cho, Kyung Sang Kim, Seung-Whan Lee, Kyu-Sun Kim, Young-Joon Jones, Walton D. Yu, Kweon |
author_sort | Suh, Yoon Seok |
collection | PubMed |
description | MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila. Through this screen, we identify miR-9a as an evolutionarily conserved regulator of insulin signalling and body growth. IPC-specific miR-9a overexpression reduces insulin signalling and body size. Of the predicted targets of miR-9a, we find that loss of miR-9a enhances the level of sNPFR1. We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. These findings indicate that the conserved miR-9a regulates body growth by controlling sNPFR1/NPYR-mediated modulation of insulin signalling. |
format | Online Article Text |
id | pubmed-4506552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45065522015-07-21 Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR Suh, Yoon Seok Bhat, Shreelatha Hong, Seung-Hyun Shin, Minjung Bahk, Suhyoung Cho, Kyung Sang Kim, Seung-Whan Lee, Kyu-Sun Kim, Young-Joon Jones, Walton D. Yu, Kweon Nat Commun Article MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila. Through this screen, we identify miR-9a as an evolutionarily conserved regulator of insulin signalling and body growth. IPC-specific miR-9a overexpression reduces insulin signalling and body size. Of the predicted targets of miR-9a, we find that loss of miR-9a enhances the level of sNPFR1. We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. These findings indicate that the conserved miR-9a regulates body growth by controlling sNPFR1/NPYR-mediated modulation of insulin signalling. Nature Pub. Group 2015-07-03 /pmc/articles/PMC4506552/ /pubmed/26138755 http://dx.doi.org/10.1038/ncomms8693 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Suh, Yoon Seok Bhat, Shreelatha Hong, Seung-Hyun Shin, Minjung Bahk, Suhyoung Cho, Kyung Sang Kim, Seung-Whan Lee, Kyu-Sun Kim, Young-Joon Jones, Walton D. Yu, Kweon Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title | Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title_full | Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title_fullStr | Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title_full_unstemmed | Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title_short | Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR |
title_sort | genome-wide microrna screening reveals that the evolutionary conserved mir-9a regulates body growth by targeting snpfr1/npyr |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506552/ https://www.ncbi.nlm.nih.gov/pubmed/26138755 http://dx.doi.org/10.1038/ncomms8693 |
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