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The Influence of Two-Dimensional Organization on Peptide Conformation**
Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solut...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506555/ https://www.ncbi.nlm.nih.gov/pubmed/25413024 http://dx.doi.org/10.1002/anie.201408971 |
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author | White, Simon J Johnson, Steven D Sellick, Mark A Bronowska, Agnieszka Stockley, Peter G Wälti, Christoph |
author_facet | White, Simon J Johnson, Steven D Sellick, Mark A Bronowska, Agnieszka Stockley, Peter G Wälti, Christoph |
author_sort | White, Simon J |
collection | PubMed |
description | Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solution. Using on-surface circular dichroism (CD) spectroscopy, we have investigated the structure of a synthetic peptide assembled into a highly packed monolayer. The immobilized peptide undergoes a structural transition between α-helical and random coil conformation upon changes in pH and ionic concentration, but critically the threshold for conformational change is altered dramatically by molecular crowding within the peptide monolayer. This study highlights the often overlooked role molecular crowding plays in regulating molecular structure and function in surface-phase studies of biological molecules. |
format | Online Article Text |
id | pubmed-4506555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-45065552015-07-22 The Influence of Two-Dimensional Organization on Peptide Conformation** White, Simon J Johnson, Steven D Sellick, Mark A Bronowska, Agnieszka Stockley, Peter G Wälti, Christoph Angew Chem Int Ed Engl Communications Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solution. Using on-surface circular dichroism (CD) spectroscopy, we have investigated the structure of a synthetic peptide assembled into a highly packed monolayer. The immobilized peptide undergoes a structural transition between α-helical and random coil conformation upon changes in pH and ionic concentration, but critically the threshold for conformational change is altered dramatically by molecular crowding within the peptide monolayer. This study highlights the often overlooked role molecular crowding plays in regulating molecular structure and function in surface-phase studies of biological molecules. WILEY-VCH Verlag 2015-01-12 2014-11-20 /pmc/articles/PMC4506555/ /pubmed/25413024 http://dx.doi.org/10.1002/anie.201408971 Text en © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications White, Simon J Johnson, Steven D Sellick, Mark A Bronowska, Agnieszka Stockley, Peter G Wälti, Christoph The Influence of Two-Dimensional Organization on Peptide Conformation** |
title | The Influence of Two-Dimensional Organization on Peptide Conformation** |
title_full | The Influence of Two-Dimensional Organization on Peptide Conformation** |
title_fullStr | The Influence of Two-Dimensional Organization on Peptide Conformation** |
title_full_unstemmed | The Influence of Two-Dimensional Organization on Peptide Conformation** |
title_short | The Influence of Two-Dimensional Organization on Peptide Conformation** |
title_sort | influence of two-dimensional organization on peptide conformation** |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506555/ https://www.ncbi.nlm.nih.gov/pubmed/25413024 http://dx.doi.org/10.1002/anie.201408971 |
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