Cargando…

The Influence of Two-Dimensional Organization on Peptide Conformation**

Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solut...

Descripción completa

Detalles Bibliográficos
Autores principales: White, Simon J, Johnson, Steven D, Sellick, Mark A, Bronowska, Agnieszka, Stockley, Peter G, Wälti, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506555/
https://www.ncbi.nlm.nih.gov/pubmed/25413024
http://dx.doi.org/10.1002/anie.201408971
_version_ 1782381707341594624
author White, Simon J
Johnson, Steven D
Sellick, Mark A
Bronowska, Agnieszka
Stockley, Peter G
Wälti, Christoph
author_facet White, Simon J
Johnson, Steven D
Sellick, Mark A
Bronowska, Agnieszka
Stockley, Peter G
Wälti, Christoph
author_sort White, Simon J
collection PubMed
description Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solution. Using on-surface circular dichroism (CD) spectroscopy, we have investigated the structure of a synthetic peptide assembled into a highly packed monolayer. The immobilized peptide undergoes a structural transition between α-helical and random coil conformation upon changes in pH and ionic concentration, but critically the threshold for conformational change is altered dramatically by molecular crowding within the peptide monolayer. This study highlights the often overlooked role molecular crowding plays in regulating molecular structure and function in surface-phase studies of biological molecules.
format Online
Article
Text
id pubmed-4506555
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher WILEY-VCH Verlag
record_format MEDLINE/PubMed
spelling pubmed-45065552015-07-22 The Influence of Two-Dimensional Organization on Peptide Conformation** White, Simon J Johnson, Steven D Sellick, Mark A Bronowska, Agnieszka Stockley, Peter G Wälti, Christoph Angew Chem Int Ed Engl Communications Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface-phase studies, in which molecular densities generally far exceed those observed in solution. Using on-surface circular dichroism (CD) spectroscopy, we have investigated the structure of a synthetic peptide assembled into a highly packed monolayer. The immobilized peptide undergoes a structural transition between α-helical and random coil conformation upon changes in pH and ionic concentration, but critically the threshold for conformational change is altered dramatically by molecular crowding within the peptide monolayer. This study highlights the often overlooked role molecular crowding plays in regulating molecular structure and function in surface-phase studies of biological molecules. WILEY-VCH Verlag 2015-01-12 2014-11-20 /pmc/articles/PMC4506555/ /pubmed/25413024 http://dx.doi.org/10.1002/anie.201408971 Text en © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
White, Simon J
Johnson, Steven D
Sellick, Mark A
Bronowska, Agnieszka
Stockley, Peter G
Wälti, Christoph
The Influence of Two-Dimensional Organization on Peptide Conformation**
title The Influence of Two-Dimensional Organization on Peptide Conformation**
title_full The Influence of Two-Dimensional Organization on Peptide Conformation**
title_fullStr The Influence of Two-Dimensional Organization on Peptide Conformation**
title_full_unstemmed The Influence of Two-Dimensional Organization on Peptide Conformation**
title_short The Influence of Two-Dimensional Organization on Peptide Conformation**
title_sort influence of two-dimensional organization on peptide conformation**
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506555/
https://www.ncbi.nlm.nih.gov/pubmed/25413024
http://dx.doi.org/10.1002/anie.201408971
work_keys_str_mv AT whitesimonj theinfluenceoftwodimensionalorganizationonpeptideconformation
AT johnsonstevend theinfluenceoftwodimensionalorganizationonpeptideconformation
AT sellickmarka theinfluenceoftwodimensionalorganizationonpeptideconformation
AT bronowskaagnieszka theinfluenceoftwodimensionalorganizationonpeptideconformation
AT stockleypeterg theinfluenceoftwodimensionalorganizationonpeptideconformation
AT waltichristoph theinfluenceoftwodimensionalorganizationonpeptideconformation
AT whitesimonj influenceoftwodimensionalorganizationonpeptideconformation
AT johnsonstevend influenceoftwodimensionalorganizationonpeptideconformation
AT sellickmarka influenceoftwodimensionalorganizationonpeptideconformation
AT bronowskaagnieszka influenceoftwodimensionalorganizationonpeptideconformation
AT stockleypeterg influenceoftwodimensionalorganizationonpeptideconformation
AT waltichristoph influenceoftwodimensionalorganizationonpeptideconformation