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Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats

BACKGROUND: Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. The aim of this study was to investigate the antidiabetic activity of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin (STZ) induced diabetic rats. METHODS: T...

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Autores principales: Toma, Alemayehu, Makonnen, Eyasu, Mekonnen, Yelamtsehay, Debella, Asfaw, Adisakwattana, Sirichai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506633/
https://www.ncbi.nlm.nih.gov/pubmed/26187590
http://dx.doi.org/10.1186/s12906-015-0779-0
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author Toma, Alemayehu
Makonnen, Eyasu
Mekonnen, Yelamtsehay
Debella, Asfaw
Adisakwattana, Sirichai
author_facet Toma, Alemayehu
Makonnen, Eyasu
Mekonnen, Yelamtsehay
Debella, Asfaw
Adisakwattana, Sirichai
author_sort Toma, Alemayehu
collection PubMed
description BACKGROUND: Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. The aim of this study was to investigate the antidiabetic activity of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin (STZ) induced diabetic rats. METHODS: The aqueous ethanol extract and n-butanol fraction of Moringa stenopetala leaves hydroalcoholic (500 mg/kg body weight) and metformin (150 mg/kg body weight) were administered to diabetic rats. Blood glucose, lipid profiles, liver and kidney function were examined after 14 days of experiment. Histopathological profile of the pancreas was also observed in diabetic rats at the end of study. An oral sucrose challenge test was also carried out to assess the post prandial effect of the extract. RESULTS: Oral administration of the aqueous ethanol and n-butanol extracts of Moringa stenopetala leaves (500 mg/kg body weight) and metformin (150 mg/kg) significantly reduced blood glucose level (P < 0.05), improved serum lipid profiles, liver enzymes and kidney functions in diabetic rats after 14 days. The extracts also improved damage of islet of Langerhan’s in diabetic rats. The plant material reduced the post-prandial glucose level (P < 0.001) at the dose of 750 mg/kg. CONCLUSION: These findings revealed that both the aqueous ethanol and n-butanol extracts of Moringa stenopetala leaves possess antihyperglycemic and antihyperlipidemic properties, and alleviate STZ-induced pancreatic damage in diabetic rats. The beneficial effects of plant material in inhibition of diabetes-induced complications are being investigated.
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spelling pubmed-45066332015-07-19 Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats Toma, Alemayehu Makonnen, Eyasu Mekonnen, Yelamtsehay Debella, Asfaw Adisakwattana, Sirichai BMC Complement Altern Med Research Article BACKGROUND: Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. The aim of this study was to investigate the antidiabetic activity of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin (STZ) induced diabetic rats. METHODS: The aqueous ethanol extract and n-butanol fraction of Moringa stenopetala leaves hydroalcoholic (500 mg/kg body weight) and metformin (150 mg/kg body weight) were administered to diabetic rats. Blood glucose, lipid profiles, liver and kidney function were examined after 14 days of experiment. Histopathological profile of the pancreas was also observed in diabetic rats at the end of study. An oral sucrose challenge test was also carried out to assess the post prandial effect of the extract. RESULTS: Oral administration of the aqueous ethanol and n-butanol extracts of Moringa stenopetala leaves (500 mg/kg body weight) and metformin (150 mg/kg) significantly reduced blood glucose level (P < 0.05), improved serum lipid profiles, liver enzymes and kidney functions in diabetic rats after 14 days. The extracts also improved damage of islet of Langerhan’s in diabetic rats. The plant material reduced the post-prandial glucose level (P < 0.001) at the dose of 750 mg/kg. CONCLUSION: These findings revealed that both the aqueous ethanol and n-butanol extracts of Moringa stenopetala leaves possess antihyperglycemic and antihyperlipidemic properties, and alleviate STZ-induced pancreatic damage in diabetic rats. The beneficial effects of plant material in inhibition of diabetes-induced complications are being investigated. BioMed Central 2015-07-18 /pmc/articles/PMC4506633/ /pubmed/26187590 http://dx.doi.org/10.1186/s12906-015-0779-0 Text en © Toma et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Toma, Alemayehu
Makonnen, Eyasu
Mekonnen, Yelamtsehay
Debella, Asfaw
Adisakwattana, Sirichai
Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title_full Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title_fullStr Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title_full_unstemmed Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title_short Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats
title_sort antidiabetic activities of aqueous ethanol and n-butanol fraction of moringa stenopetala leaves in streptozotocin-induced diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506633/
https://www.ncbi.nlm.nih.gov/pubmed/26187590
http://dx.doi.org/10.1186/s12906-015-0779-0
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