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Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats

Reduced beta cell mass in pancreatic islets (PI) of Goto-Kakizaki (GK) rats is frequently observed in this diabetic model, but knowledge on delta cells is scarce. Aiming to compare delta cell physiology/pathology of GK to Wistar rats, we found that delta cell number increased over time as did somato...

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Autores principales: Alán, Lukáš, Olejár, Tomáš, Cahová, Monika, Zelenka, Jaroslav, Berková, Zuzana, Smětáková, Magdalena, Saudek, František, Matěj, Radoslav, Ježek, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506919/
https://www.ncbi.nlm.nih.gov/pubmed/26236746
http://dx.doi.org/10.1155/2015/385395
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author Alán, Lukáš
Olejár, Tomáš
Cahová, Monika
Zelenka, Jaroslav
Berková, Zuzana
Smětáková, Magdalena
Saudek, František
Matěj, Radoslav
Ježek, Petr
author_facet Alán, Lukáš
Olejár, Tomáš
Cahová, Monika
Zelenka, Jaroslav
Berková, Zuzana
Smětáková, Magdalena
Saudek, František
Matěj, Radoslav
Ježek, Petr
author_sort Alán, Lukáš
collection PubMed
description Reduced beta cell mass in pancreatic islets (PI) of Goto-Kakizaki (GK) rats is frequently observed in this diabetic model, but knowledge on delta cells is scarce. Aiming to compare delta cell physiology/pathology of GK to Wistar rats, we found that delta cell number increased over time as did somatostatin mRNA and delta cells distribution in PI is different in GK rats. Subtle changes in 6-week-old GK rats were found. With maturation and aging of GK rats, disturbed cytoarchitecture occurred with irregular beta cells accompanied by delta cell hyperplasia and loss of pancreatic polypeptide (PPY) positivity. Unlike the constant glucose-stimulation index for insulin PI release in Wistar rats, this index declined with GK age, whereas for somatostatin it increased with age. A decrease of GK rat PPY serum levels was found. GK rat body weight decreased with increasing hyperglycemia. Somatostatin analog octreotide completely blocked insulin secretion, impaired proliferation at low autocrine insulin, and decreased PPY secretion and mitochondrial DNA in INS-1E cells. In conclusion, in GK rats PI, significant local delta cell hyperplasia and suspected paracrine effect of somatostatin diminish beta cell viability and contribute to the deterioration of beta cell mass. Altered PPY-secreting cells distribution amends another component of GK PI's pathophysiology.
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spelling pubmed-45069192015-08-02 Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats Alán, Lukáš Olejár, Tomáš Cahová, Monika Zelenka, Jaroslav Berková, Zuzana Smětáková, Magdalena Saudek, František Matěj, Radoslav Ježek, Petr J Diabetes Res Research Article Reduced beta cell mass in pancreatic islets (PI) of Goto-Kakizaki (GK) rats is frequently observed in this diabetic model, but knowledge on delta cells is scarce. Aiming to compare delta cell physiology/pathology of GK to Wistar rats, we found that delta cell number increased over time as did somatostatin mRNA and delta cells distribution in PI is different in GK rats. Subtle changes in 6-week-old GK rats were found. With maturation and aging of GK rats, disturbed cytoarchitecture occurred with irregular beta cells accompanied by delta cell hyperplasia and loss of pancreatic polypeptide (PPY) positivity. Unlike the constant glucose-stimulation index for insulin PI release in Wistar rats, this index declined with GK age, whereas for somatostatin it increased with age. A decrease of GK rat PPY serum levels was found. GK rat body weight decreased with increasing hyperglycemia. Somatostatin analog octreotide completely blocked insulin secretion, impaired proliferation at low autocrine insulin, and decreased PPY secretion and mitochondrial DNA in INS-1E cells. In conclusion, in GK rats PI, significant local delta cell hyperplasia and suspected paracrine effect of somatostatin diminish beta cell viability and contribute to the deterioration of beta cell mass. Altered PPY-secreting cells distribution amends another component of GK PI's pathophysiology. Hindawi Publishing Corporation 2015 2015-07-06 /pmc/articles/PMC4506919/ /pubmed/26236746 http://dx.doi.org/10.1155/2015/385395 Text en Copyright © 2015 Lukáš Alán et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Alán, Lukáš
Olejár, Tomáš
Cahová, Monika
Zelenka, Jaroslav
Berková, Zuzana
Smětáková, Magdalena
Saudek, František
Matěj, Radoslav
Ježek, Petr
Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title_full Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title_fullStr Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title_full_unstemmed Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title_short Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats
title_sort delta cell hyperplasia in adult goto-kakizaki (gk/moltac) diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506919/
https://www.ncbi.nlm.nih.gov/pubmed/26236746
http://dx.doi.org/10.1155/2015/385395
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