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Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat

Aims. To measure the expression of activin βA-subunit, activin IIA and IIB receptors, Smad4, Smad7, and follistatin in the liver and the liver and serum concentrations of mature activin-A and follistatin in normal rat following treatment with pegylated interferon-α (Peg-INF-α) and ribavirin (RBV). M...

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Autores principales: Refaat, Bassem, El-Shemi, Adel Galal, Ashshi, Ahmed Mohamed, Mahamid, Elaf Wael, Al-Qadi, Noha Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506924/
https://www.ncbi.nlm.nih.gov/pubmed/26236109
http://dx.doi.org/10.1155/2015/414207
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author Refaat, Bassem
El-Shemi, Adel Galal
Ashshi, Ahmed Mohamed
Mahamid, Elaf Wael
Al-Qadi, Noha Mohammed
author_facet Refaat, Bassem
El-Shemi, Adel Galal
Ashshi, Ahmed Mohamed
Mahamid, Elaf Wael
Al-Qadi, Noha Mohammed
author_sort Refaat, Bassem
collection PubMed
description Aims. To measure the expression of activin βA-subunit, activin IIA and IIB receptors, Smad4, Smad7, and follistatin in the liver and the liver and serum concentrations of mature activin-A and follistatin in normal rat following treatment with pegylated interferon-α (Peg-INF-α) and ribavirin (RBV). Materials and Methods. 40 male Wistar rats were divided equally into 4 groups: “control,” “Peg-only” receiving 4 injections of Peg-INF-α (6 µg/rat/week), “RBV-only” receiving ribavirin (4 mg/rat/day) orally, and “Peg & RBV” group receiving both drugs. The expression of candidate molecules in liver was measured by immunohistochemistry and quantitative PCR. The concentrations of mature proteins in serum and liver homogenate samples were measured using ELISA. Results. Peg-INF-α  ± RBV altered the expression of all candidate molecules in the liver at the gene and protein levels (P < 0.05) and decreased activin-A and increased follistatin in serum and liver homogenates compared with the other groups (P < 0.05). There were also significant correlations between serum and liver activin-A and follistatin. Conclusion. Peg-INF-α modulates the hepatic production of activin-A and follistatin, which can be detected in serum. Further studies are needed to explore the role of Peg-INF-α on the production of activins and follistatin by the liver and immune cells.
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spelling pubmed-45069242015-08-02 Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat Refaat, Bassem El-Shemi, Adel Galal Ashshi, Ahmed Mohamed Mahamid, Elaf Wael Al-Qadi, Noha Mohammed Mediators Inflamm Research Article Aims. To measure the expression of activin βA-subunit, activin IIA and IIB receptors, Smad4, Smad7, and follistatin in the liver and the liver and serum concentrations of mature activin-A and follistatin in normal rat following treatment with pegylated interferon-α (Peg-INF-α) and ribavirin (RBV). Materials and Methods. 40 male Wistar rats were divided equally into 4 groups: “control,” “Peg-only” receiving 4 injections of Peg-INF-α (6 µg/rat/week), “RBV-only” receiving ribavirin (4 mg/rat/day) orally, and “Peg & RBV” group receiving both drugs. The expression of candidate molecules in liver was measured by immunohistochemistry and quantitative PCR. The concentrations of mature proteins in serum and liver homogenate samples were measured using ELISA. Results. Peg-INF-α  ± RBV altered the expression of all candidate molecules in the liver at the gene and protein levels (P < 0.05) and decreased activin-A and increased follistatin in serum and liver homogenates compared with the other groups (P < 0.05). There were also significant correlations between serum and liver activin-A and follistatin. Conclusion. Peg-INF-α modulates the hepatic production of activin-A and follistatin, which can be detected in serum. Further studies are needed to explore the role of Peg-INF-α on the production of activins and follistatin by the liver and immune cells. Hindawi Publishing Corporation 2015 2015-07-06 /pmc/articles/PMC4506924/ /pubmed/26236109 http://dx.doi.org/10.1155/2015/414207 Text en Copyright © 2015 Bassem Refaat et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Refaat, Bassem
El-Shemi, Adel Galal
Ashshi, Ahmed Mohamed
Mahamid, Elaf Wael
Al-Qadi, Noha Mohammed
Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title_full Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title_fullStr Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title_full_unstemmed Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title_short Pegylated Interferon-α Modulates Liver Concentrations of Activin-A and Its Related Proteins in Normal Wistar Rat
title_sort pegylated interferon-α modulates liver concentrations of activin-a and its related proteins in normal wistar rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506924/
https://www.ncbi.nlm.nih.gov/pubmed/26236109
http://dx.doi.org/10.1155/2015/414207
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