Cargando…

Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells

Morus alba root extract (MARE) has been used to treat hyperglycaemic conditions in oriental medicine. Here, we studied whether MARE possesses a cytotoxic effect on neuroblastoma. To check the cytotoxicity generated by MARE was whether relatively higher against the cancer cells rather than normal cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Kwon, Young Hwi, Bishayee, Kausik, Rahman, Ataur, Hong, Jae Seung, Lim, Soon-Sung, Huh, Sung-Oh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507029/
https://www.ncbi.nlm.nih.gov/pubmed/25921607
http://dx.doi.org/10.14348/molcells.2015.0030
_version_ 1782381761824555008
author Kwon, Young Hwi
Bishayee, Kausik
Rahman, Ataur
Hong, Jae Seung
Lim, Soon-Sung
Huh, Sung-Oh
author_facet Kwon, Young Hwi
Bishayee, Kausik
Rahman, Ataur
Hong, Jae Seung
Lim, Soon-Sung
Huh, Sung-Oh
author_sort Kwon, Young Hwi
collection PubMed
description Morus alba root extract (MARE) has been used to treat hyperglycaemic conditions in oriental medicine. Here, we studied whether MARE possesses a cytotoxic effect on neuroblastoma. To check the cytotoxicity generated by MARE was whether relatively higher against the cancer cells rather than normal cells, we chose a neuroblastoma cell line (B103) and a normal cell line (Rat-2). A CCK assay revealed that MARE (10 μg/ml) reduced cell viability to approximately 60% compared to an untreated control in B103 cells. But in Rat-2 cells, MARE induced relatively lower cytotoxicity. To investigate the mechanisms underlying the cytotoxic effect of MARE, we used flow cytometry combined with immunoblot analyses. We found that MARE-treatment could accumulate ROS and depolarize mitochondria membrane potential of B103 cells. Further treatment with MARE in B103 cells also could damage DNA and induce apoptosis. An expression study of p-Akt also suggested that there was a reduction in cellular proliferation and transcription along with the process of apoptosis, which was further evidenced by an increase in Bax and cleaved-caspase 3 activity. Together, our findings suggest that MARE produces more cytotoxicity in cancer cells while having a relatively attenuated effect on normal cells. As such, MARE may be a safer option in cancer therapeutics, and it also shows potential for the patients with symptoms of hyperglycemia and cancer.
format Online
Article
Text
id pubmed-4507029
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Korean Society for Molecular and Cellular Biology
record_format MEDLINE/PubMed
spelling pubmed-45070292015-07-24 Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells Kwon, Young Hwi Bishayee, Kausik Rahman, Ataur Hong, Jae Seung Lim, Soon-Sung Huh, Sung-Oh Mol Cells Article Morus alba root extract (MARE) has been used to treat hyperglycaemic conditions in oriental medicine. Here, we studied whether MARE possesses a cytotoxic effect on neuroblastoma. To check the cytotoxicity generated by MARE was whether relatively higher against the cancer cells rather than normal cells, we chose a neuroblastoma cell line (B103) and a normal cell line (Rat-2). A CCK assay revealed that MARE (10 μg/ml) reduced cell viability to approximately 60% compared to an untreated control in B103 cells. But in Rat-2 cells, MARE induced relatively lower cytotoxicity. To investigate the mechanisms underlying the cytotoxic effect of MARE, we used flow cytometry combined with immunoblot analyses. We found that MARE-treatment could accumulate ROS and depolarize mitochondria membrane potential of B103 cells. Further treatment with MARE in B103 cells also could damage DNA and induce apoptosis. An expression study of p-Akt also suggested that there was a reduction in cellular proliferation and transcription along with the process of apoptosis, which was further evidenced by an increase in Bax and cleaved-caspase 3 activity. Together, our findings suggest that MARE produces more cytotoxicity in cancer cells while having a relatively attenuated effect on normal cells. As such, MARE may be a safer option in cancer therapeutics, and it also shows potential for the patients with symptoms of hyperglycemia and cancer. Korean Society for Molecular and Cellular Biology 2015-07-31 2015-07-03 /pmc/articles/PMC4507029/ /pubmed/25921607 http://dx.doi.org/10.14348/molcells.2015.0030 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Kwon, Young Hwi
Bishayee, Kausik
Rahman, Ataur
Hong, Jae Seung
Lim, Soon-Sung
Huh, Sung-Oh
Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title_full Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title_fullStr Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title_full_unstemmed Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title_short Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells
title_sort morus alba accumulates reactive oxygen species to initiate apoptosis via foxo-caspase 3-dependent pathway in neuroblastoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507029/
https://www.ncbi.nlm.nih.gov/pubmed/25921607
http://dx.doi.org/10.14348/molcells.2015.0030
work_keys_str_mv AT kwonyounghwi morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells
AT bishayeekausik morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells
AT rahmanataur morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells
AT hongjaeseung morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells
AT limsoonsung morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells
AT huhsungoh morusalbaaccumulatesreactiveoxygenspeciestoinitiateapoptosisviafoxocaspase3dependentpathwayinneuroblastomacells