Cargando…
Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds
Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507089/ https://www.ncbi.nlm.nih.gov/pubmed/25923923 http://dx.doi.org/10.1089/ten.tea.2014.0485 |
_version_ | 1782381768220868608 |
---|---|
author | El-Gendy, Reem Kirkham, Jennifer Newby, Phillipa J. Mohanram, Yamuna Boccaccini, Aldo Roberto Yang, Xuebin B. |
author_facet | El-Gendy, Reem Kirkham, Jennifer Newby, Phillipa J. Mohanram, Yamuna Boccaccini, Aldo Roberto Yang, Xuebin B. |
author_sort | El-Gendy, Reem |
collection | PubMed |
description | Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass(®) scaffolds for tissue-engineered mineral constructs in vivo and in vitro. 45S5 Bioglass scaffolds were produced by the foam replication technique with the standard composition of 45 wt% SiO(2), 24.5 wt% Na(2)O, 24.5 wt% CaO, and 6 wt% P(2)O(5). HDPSCs were cultured in monolayers and on porous 45S5 Bioglass scaffolds under angiogenic and osteogenic conditions for 2–4 weeks. HDPSCs expressed endothelial gene markers (CD34, CD31/PECAM1, and VEGFR2) under both conditions in the monolayer. A combination of HDPSCs with 45S5 Bioglass enhanced the expression of these gene markers. Positive immunostaining for CD31/PECAM1 and VEGFR2 and negative staining for CD34 supported the gene expression data, while histology revealed evidence of endothelial cell-like morphology within the constructs. More organized tubular structures, resembling microvessels, were seen in the constructs after 8 weeks of implantation in vivo. In conclusion, this study suggests that the combination of HDPSCs with 45S5 Bioglass scaffolds offers a promising strategy for regenerating vascularized bone grafts. |
format | Online Article Text |
id | pubmed-4507089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Mary Ann Liebert, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45070892015-09-23 Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds El-Gendy, Reem Kirkham, Jennifer Newby, Phillipa J. Mohanram, Yamuna Boccaccini, Aldo Roberto Yang, Xuebin B. Tissue Eng Part A Original Articles Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass(®) scaffolds for tissue-engineered mineral constructs in vivo and in vitro. 45S5 Bioglass scaffolds were produced by the foam replication technique with the standard composition of 45 wt% SiO(2), 24.5 wt% Na(2)O, 24.5 wt% CaO, and 6 wt% P(2)O(5). HDPSCs were cultured in monolayers and on porous 45S5 Bioglass scaffolds under angiogenic and osteogenic conditions for 2–4 weeks. HDPSCs expressed endothelial gene markers (CD34, CD31/PECAM1, and VEGFR2) under both conditions in the monolayer. A combination of HDPSCs with 45S5 Bioglass enhanced the expression of these gene markers. Positive immunostaining for CD31/PECAM1 and VEGFR2 and negative staining for CD34 supported the gene expression data, while histology revealed evidence of endothelial cell-like morphology within the constructs. More organized tubular structures, resembling microvessels, were seen in the constructs after 8 weeks of implantation in vivo. In conclusion, this study suggests that the combination of HDPSCs with 45S5 Bioglass scaffolds offers a promising strategy for regenerating vascularized bone grafts. Mary Ann Liebert, Inc. 2015-07-01 2015-04-27 /pmc/articles/PMC4507089/ /pubmed/25923923 http://dx.doi.org/10.1089/ten.tea.2014.0485 Text en © R. El-Gendy et al. 2015; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Original Articles El-Gendy, Reem Kirkham, Jennifer Newby, Phillipa J. Mohanram, Yamuna Boccaccini, Aldo Roberto Yang, Xuebin B. Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title | Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title_full | Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title_fullStr | Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title_full_unstemmed | Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title_short | Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass(®) Scaffolds |
title_sort | investigating the vascularization of tissue-engineered bone constructs using dental pulp cells and 45s5 bioglass(®) scaffolds |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507089/ https://www.ncbi.nlm.nih.gov/pubmed/25923923 http://dx.doi.org/10.1089/ten.tea.2014.0485 |
work_keys_str_mv | AT elgendyreem investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds AT kirkhamjennifer investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds AT newbyphillipaj investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds AT mohanramyamuna investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds AT boccaccinialdoroberto investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds AT yangxuebinb investigatingthevascularizationoftissueengineeredboneconstructsusingdentalpulpcellsand45s5bioglassscaffolds |