Cargando…

Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB

DNA phosphorothioate (PT) modification, in which the non-bridging oxygen of the sugar-phosphate backbone is substituted by sulfur, occurs naturally in diverse bacteria and archaea and is regulated by the DndABCDE proteins. DndABCDE and the restriction cognate DndFGHI constitute a new type of defense...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Wei, Huang, Teng, Tang, You, Liu, Yanhua, Wu, Xiaolin, Chen, Si, Chan, Wan, Wang, Yajie, Liu, Xiaoyun, Chen, Shi, Wang, Lianrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507180/
https://www.ncbi.nlm.nih.gov/pubmed/26190504
http://dx.doi.org/10.1038/srep12368
_version_ 1782381774768177152
author He, Wei
Huang, Teng
Tang, You
Liu, Yanhua
Wu, Xiaolin
Chen, Si
Chan, Wan
Wang, Yajie
Liu, Xiaoyun
Chen, Shi
Wang, Lianrong
author_facet He, Wei
Huang, Teng
Tang, You
Liu, Yanhua
Wu, Xiaolin
Chen, Si
Chan, Wan
Wang, Yajie
Liu, Xiaoyun
Chen, Shi
Wang, Lianrong
author_sort He, Wei
collection PubMed
description DNA phosphorothioate (PT) modification, in which the non-bridging oxygen of the sugar-phosphate backbone is substituted by sulfur, occurs naturally in diverse bacteria and archaea and is regulated by the DndABCDE proteins. DndABCDE and the restriction cognate DndFGHI constitute a new type of defense system that prevents the invasion of foreign DNA in Salmonella enterica serovar Cerro 87. GAAC/GTTC consensus contexts across genomes were found to possess partial PT modifications even in the presence of restriction activity, indicating the regulation of PT. The abundance of PT in cells must be controlled to suit cellular activities. However, the regulatory mechanism of PT modification has not been characterized. The result here indicated that genomic PT modification in S. enterica is controlled by the transcriptional regulator DndB, which binds to two regions in the promoter, each possessing a 5′-TACGN(10)CGTA-3′ palindromic motif, to regulate the transcription of dndCDE and its own gene. Site-directed mutagenesis showed that the Cys29 residue of DndB plays a key role in its DNA-binding activity or conformation. Proteomic analysis identified changes to a number of cellular proteins upon up-regulation and loss of PT. Considering the genetic conservation of dnd operons, regulation of PT by DndB might be widespread in diverse organisms.
format Online
Article
Text
id pubmed-4507180
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-45071802015-07-21 Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB He, Wei Huang, Teng Tang, You Liu, Yanhua Wu, Xiaolin Chen, Si Chan, Wan Wang, Yajie Liu, Xiaoyun Chen, Shi Wang, Lianrong Sci Rep Article DNA phosphorothioate (PT) modification, in which the non-bridging oxygen of the sugar-phosphate backbone is substituted by sulfur, occurs naturally in diverse bacteria and archaea and is regulated by the DndABCDE proteins. DndABCDE and the restriction cognate DndFGHI constitute a new type of defense system that prevents the invasion of foreign DNA in Salmonella enterica serovar Cerro 87. GAAC/GTTC consensus contexts across genomes were found to possess partial PT modifications even in the presence of restriction activity, indicating the regulation of PT. The abundance of PT in cells must be controlled to suit cellular activities. However, the regulatory mechanism of PT modification has not been characterized. The result here indicated that genomic PT modification in S. enterica is controlled by the transcriptional regulator DndB, which binds to two regions in the promoter, each possessing a 5′-TACGN(10)CGTA-3′ palindromic motif, to regulate the transcription of dndCDE and its own gene. Site-directed mutagenesis showed that the Cys29 residue of DndB plays a key role in its DNA-binding activity or conformation. Proteomic analysis identified changes to a number of cellular proteins upon up-regulation and loss of PT. Considering the genetic conservation of dnd operons, regulation of PT by DndB might be widespread in diverse organisms. Nature Publishing Group 2015-07-20 /pmc/articles/PMC4507180/ /pubmed/26190504 http://dx.doi.org/10.1038/srep12368 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
He, Wei
Huang, Teng
Tang, You
Liu, Yanhua
Wu, Xiaolin
Chen, Si
Chan, Wan
Wang, Yajie
Liu, Xiaoyun
Chen, Shi
Wang, Lianrong
Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title_full Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title_fullStr Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title_full_unstemmed Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title_short Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
title_sort regulation of dna phosphorothioate modification in salmonella enterica by dndb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507180/
https://www.ncbi.nlm.nih.gov/pubmed/26190504
http://dx.doi.org/10.1038/srep12368
work_keys_str_mv AT hewei regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT huangteng regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT tangyou regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT liuyanhua regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT wuxiaolin regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT chensi regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT chanwan regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT wangyajie regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT liuxiaoyun regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT chenshi regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb
AT wanglianrong regulationofdnaphosphorothioatemodificationinsalmonellaentericabydndb