Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high eff...

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Detalles Bibliográficos
Autores principales: Wang, Zhi-Ping, Xing, Hui-Li, Dong, Li, Zhang, Hai-Yan, Han, Chun-Yan, Wang, Xue-Chen, Chen, Qi-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507317/
https://www.ncbi.nlm.nih.gov/pubmed/26193878
http://dx.doi.org/10.1186/s13059-015-0715-0

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