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Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts

Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São...

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Autores principales: Oliveira, Lilian C.G., Ramos, Patricia Locosque, Marem, Alyne, Kondo, Marcia Y., Rocha, Rafael C.S., Bertolini, Thiago, Silveira, Marghuel A.V., da Cruz, João Batista, de Vasconcellos, Suzan Pantaroto, Juliano, Luiz, Okamoto, Debora N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507525/
https://www.ncbi.nlm.nih.gov/pubmed/26273248
http://dx.doi.org/10.1590/S1517-838246220130316
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author Oliveira, Lilian C.G.
Ramos, Patricia Locosque
Marem, Alyne
Kondo, Marcia Y.
Rocha, Rafael C.S.
Bertolini, Thiago
Silveira, Marghuel A.V.
da Cruz, João Batista
de Vasconcellos, Suzan Pantaroto
Juliano, Luiz
Okamoto, Debora N.
author_facet Oliveira, Lilian C.G.
Ramos, Patricia Locosque
Marem, Alyne
Kondo, Marcia Y.
Rocha, Rafael C.S.
Bertolini, Thiago
Silveira, Marghuel A.V.
da Cruz, João Batista
de Vasconcellos, Suzan Pantaroto
Juliano, Luiz
Okamoto, Debora N.
author_sort Oliveira, Lilian C.G.
collection PubMed
description Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications.
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spelling pubmed-45075252015-08-13 Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts Oliveira, Lilian C.G. Ramos, Patricia Locosque Marem, Alyne Kondo, Marcia Y. Rocha, Rafael C.S. Bertolini, Thiago Silveira, Marghuel A.V. da Cruz, João Batista de Vasconcellos, Suzan Pantaroto Juliano, Luiz Okamoto, Debora N. Braz J Microbiol Environmental Microbiology Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications. Sociedade Brasileira de Microbiologia 2015-06-01 /pmc/articles/PMC4507525/ /pubmed/26273248 http://dx.doi.org/10.1590/S1517-838246220130316 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Environmental Microbiology
Oliveira, Lilian C.G.
Ramos, Patricia Locosque
Marem, Alyne
Kondo, Marcia Y.
Rocha, Rafael C.S.
Bertolini, Thiago
Silveira, Marghuel A.V.
da Cruz, João Batista
de Vasconcellos, Suzan Pantaroto
Juliano, Luiz
Okamoto, Debora N.
Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title_full Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title_fullStr Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title_full_unstemmed Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title_short Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
title_sort halotolerant bacteria in the são paulo zoo composting process and their hydrolases and bioproducts
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507525/
https://www.ncbi.nlm.nih.gov/pubmed/26273248
http://dx.doi.org/10.1590/S1517-838246220130316
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